ArticlePloS one2012
High confidence prediction of essential genes in Burkholderia cenocepacia.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
32 citing papers in PubMed, 59 citations in OpenAlex.
- Identification of discriminant features from stationary pattern of nucleotide bases and their application to essential gene classification.Frontiers in genetics · 2023Article
- Bacterial genome reductions: Tools, applications, and challenges.Frontiers in genome editing · 2022Review
- Targeting the Nonmevalonate Pathway in Burkholderia cenocepacia Increases Susceptibility to Certain β-Lactam Antibiotics.Antimicrobial agents and chemotherapy · 2018Article
- Variability of Bacterial Essential Genes Among Closely Related Bacteria: The Case ofFrontiers in microbiology · 2018Review
- Genetic Determinants Associated WithFrontiers in microbiology · 2018Article
- The Essential Genome of Burkholderia cenocepacia H111.Journal of bacteriology · 2017Article
- Comparative analysis of the Burkholderia cenocepacia K56-2 essential genome reveals cell envelope functions that are uniquely required for survival in species of the genus Burkholderia.Microbial genomics · 2017Article
- Use of Synthetic Hybrid Strains To Determine the Role of Replicon 3 in Virulence of the Burkholderia cepacia Complex.Applied and environmental microbiology · 2017Article
- Identification and analysis of genomic islands in Burkholderia cenocepacia AU 1054 with emphasis on pathogenicity islands.BMC microbiology · 2017Article
- The Escherichia coli rhaSR-PrhaBAD Inducible Promoter System Allows Tightly Controlled Gene Expression over a Wide Range in Pseudomonas aeruginosa.Applied and environmental microbiology · 2016Article
- Predicting Essential Genes and Proteins Based on Machine Learning and Network Topological Features: A Comprehensive Review.Frontiers in physiology · 2016Review
- Candidate Essential Genes in Burkholderia cenocepacia J2315 Identified by Genome-Wide TraDIS.Frontiers in microbiology · 2016Article
- Genome-wide transcription start site profiling in biofilm-grown Burkholderia cenocepacia J2315.BMC genomics · 2015Article
- Draft Genome Sequences of Burkholderia contaminans, a Burkholderia cepacia Complex Species That Is Increasingly Recovered from Cystic Fibrosis Patients.Genome announcements · 2015Article
- Comparative analysis of essential genes in prokaryotic genomic islands.Scientific reports · 2015Article
- Flagellar region 3b supports strong expression of integrated DNA and the highest chromosomal integration efficiency of the Escherichia coli flagellar regions.Microbial biotechnology · 2015Article
- Identification and validation of novel chromosomal integration and expression loci in Escherichia coli flagellar region 1.PloS one · 2015Article
- Identification of the flagellin glycosylation system in Burkholderia cenocepacia and the contribution of glycosylated flagellin to evasion of human innate immune responses.The Journal of biological chemistry · 2014Article
- The complete genome of Burkholderia phenoliruptrix strain BR3459a, a symbiont of Mimosa flocculosa: highlighting the coexistence of symbiotic and pathogenic genes.BMC genomics · 2014Article
- The third replicon of members of the Burkholderia cepacia Complex, plasmid pC3, plays a role in stress tolerance.Applied and environmental microbiology · 2014Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 5 countries.
Funding
Abstract
backgroundEssential genes are absolutely required for the survival of an organism. The identification of essential genes, besides being one of the most fundamental questions in biology, is also of interest for the emerging science of synthetic biology and for the development of novel antimicrobials. New antimicrobial therapies are desperately needed to treat multidrug-resistant pathogens, such as members of the Burkholderia cepacia complex. METHODOLOGY/PRINCIPAL
findingsWe hypothesize that essential genes may be highly conserved within a group of evolutionary closely related organisms. Using a bioinformatics approach we determined that the core genome of the order Burkholderiales consists of 649 genes. All but two of these identified genes were located on chromosome 1 of Burkholderia cenocepacia. Although many of the 649 core genes of Burkholderiales have been shown to be essential in other bacteria, we were also able to identify a number of novel essential genes present mainly, or exclusively, within this order. The essentiality of some of the core genes, including the known essential genes infB, gyrB, ubiB, and valS, as well as the so far uncharacterized genes BCAL1882, BCAL2769, BCAL3142 and BCAL3369 has been confirmed experimentally in B. cenocepacia. CONCLUSIONS/SIGNIFICANCE: We report on the identification of essential genes using a novel bioinformatics strategy and provide bioinformatics and experimental evidence that the large majority of the identified genes are indeed essential. The essential genes identified here may represent valuable targets for the development of novel antimicrobials and their detailed study may shed new light on the functions required to support life.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.