ArticlePloS one2012
Dimethyl sulfoxide induces both direct and indirect tau hyperphosphorylation.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Temperature-Dependent Aggregation of Tau Protein Is Attenuated by Native PLGA Nanoparticles Under in vitro Conditions.International journal of nanomedicine · 2025Article
- Temperature-induced Artifacts in Tau Phosphorylation: Implications for Reliable Alzheimer's Disease Research.Experimental neurobiology · 2023Article
- DMSO potentiates the suppressive effect of dronabinol on sleep apnea and REM sleep in rats.Journal of cannabis research · 2023Article
- Histopathological evaluation of insulin-DMSO formula designed for direct nose-to-brain delivery.Histology and histopathology · 2022Article
- Heterogeneous Off-Target Effects of Ultra-Low Dose Dimethyl Sulfoxide (DMSO) on Targetable Signaling Events in Lung Cancer In Vitro Models.International journal of molecular sciences · 2021Article
- Development and evaluation of [Synapse (New York, N.Y.) · 2021Article
- Bovine Fetal Mesenchymal Stem Cells Obtained From Omental Adipose Tissue and Placenta Are More Resistant to Cryoprotectant Exposure Than Those From Bone Marrow.Frontiers in veterinary science · 2021Article
- A Rational Insight into the Effect of Dimethyl Sulfoxide on TNF-α Activity.International journal of molecular sciences · 2020Article
- Microglial depletion prevents extracellular matrix changes and striatal volume reduction in a model of Huntington's disease.Brain : a journal of neurology · 2020Article
- Blockade of 5-HT2A receptors inhibits emotional hyperthermia in mice.The journal of physiological sciences : JPS · 2019Article
- Istradefylline reduces memory deficits in aging mice with amyloid pathology.Neurobiology of disease · 2018Article
- Insulin deprivation induces PP2A inhibition and tau hyperphosphorylation in hTau mice, a model of Alzheimer's disease-like tau pathology.Scientific reports · 2017Article
- In situ fibrillizing amyloid-beta 1-42 induces neurite degeneration and apoptosis of differentiated SH-SY5Y cells.PloS one · 2017Article
- Dynamic Regulation of AMPAR Phosphorylation In Vivo Following Acute Behavioral Stress.Cellular and molecular neurobiology · 2016Article
- The Co-chaperone BAG2 Mediates Cold-Induced Accumulation of Phosphorylated Tau in SH-SY5Y Cells.Cellular and molecular neurobiology · 2016Article
- Dimethyl sulfoxide damages mitochondrial integrity and membrane potential in cultured astrocytes.PloS one · 2014Article
- Neurogenesis is required for behavioral recovery after injury in the visual system of Xenopus laevis.The Journal of comparative neurology · 2013Article
- Temperature and toxic Tau in Alzheimer's disease: new insights.Temperature (Austin, Tex.)Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Dimethyl sulfoxide (DMSO) is widely used as a solvent or vehicle for biological studies, and for treatment of specific disorders, including traumatic brain injury and several forms of amyloidosis. As Alzheimer's disease (AD) brains are characterized by deposits of β-amyloid peptides, it has been suggested that DMSO could be used as a treatment for this devastating disease. AD brains are also characterized by aggregates of hyperphosphorylated tau protein, but the effect of DMSO on tau phosphorylation is unknown. We thus investigated the impact of DMSO on tau phosphorylation in vitro and in vivo. One hour following intraperitoneal administration of 1 or 2 ml/kg DMSO in mice, no change was observed in tau phosphorylation. However, at 4 ml/kg, tau was hyperphosphorylated at AT8 (Ser(202)/Thr(205)), PHF-1 (Ser(396)/Ser(404)) and AT180 (Thr(231)) epitopes. At this dose, we also noticed that the animals were hypothermic. When the mice were maintained normothermic, the effect of 4 ml/kg DMSO on tau hyperphosphorylation was prevented. On the other hand, in SH-SY5Y cells, 0.1% DMSO induced tau hyperphosphorylation at AT8 and AT180 phosphoepitopes in normothermic conditions. Globally, these findings demonstrate that DMSO can induce tau hyperphosphorylation indirectly via hypothermia in vivo, and directly in vitro. These data should caution researchers working with DMSO as it can induce artifactual results both in vivo and in vitro.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.