Evidence map›Paper›PMID 22767600›Full record

ArticleThe Journal of biological chemistry2012

Genetic engineering of the phosphocarrier protein NPr of the Escherichia coli phosphotransferase system selectively improves sugar uptake activity.

Yossef Lopez-de Los Santos, Henry Chan, Vito A Cantu, Rachael Rettner, Filiberto Sanchez, Zhongge Zhang, Milton H Saier, Xavier Soberon

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Yossef Lopez-de Los SantosDepartamento de Ingeniería Celular y Biocatálisis del Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenue Universidad 2001, Cuernavaca, Morelos, México, P. C. 62210.
Henry Chan
Vito A Cantu
Rachael Rettner
Filiberto Sanchez
Zhongge Zhang
Milton H Saier
Xavier Soberon
University of California, San Diego · USUniversidad Nacional Autónoma de México · MXUniversidad Autónoma del Estado de Morelos · MX

Funding

The Transporter Classification Database (TCDB)R01GM077402 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SAIER, MILTON H. · 2006 to 2021
$4.7M
NIGMS NIH HHS GM077402NIGMS NIH HHS R01 GM077402
6 · The paper itself

Abstract

The Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system (PTS) in prokaryotes mediates the uptake and phosphorylation of its numerous substrates through a phosphoryl transfer chain where a phosphoryl transfer protein, HPr, transfers its phosphoryl group to any of several sugar-specific Enzyme IIA proteins in preparation for sugar transport. A phosphoryl transfer protein of the PTS, NPr, homologous to HPr, functions to regulate nitrogen metabolism and shows virtually no enzymatic cross-reactivity with HPr. Here we describe the genetic engineering of a "chimeric" HPr/NPr protein, termed CPr14 because 14 amino acid residues of the interface were replaced. CPr14 shows decreased activity with most PTS permeases relative to HPr, but increases activity with the broad specificity mannose permease. The results lead to the proposal that HPr is not optimal for most PTS permeases but instead represents a compromise with suboptimal activity for most PTS permeases. The evolutionary implications are discussed.

Indexed as

Genetic EngineeringBacterial ProteinsCarrier ProteinsEscherichia coliEscherichia coli ProteinsPhosphate-Binding ProteinsPhosphoenolpyruvate Sugar Phosphotransferase SystemRecombinant Fusion ProteinsSubstrate SpecificityBacterial ProteinsCarrier ProteinsEscherichia coli ProteinsNPr protein, E coliPhosphate-Binding Proteinsphosphocarrier protein HPrphosphoenolpyruvate-mannose phosphotransferasePhosphoenolpyruvate Sugar Phosphotransferase SystemRecombinant Fusion Proteins

Identifiers

PMID22767600
PMCPMC3436175
OpenAlexW2074249867

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.