ArticleMolecular biology of the cell2012
Septin ring size scaling and dynamics require the coiled-coil region of Shs1p.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Septins associate with AP-3 to support trafficking to the vacuole/lysosome in yeast.Molecular biology of the cell · 2026Article
- Septins associate with AP-3 to support trafficking to the vacuole/lysosome in yeast.bioRxiv : the preprint server for biology · 2026Article
- ECM-dependent regulation of septin 7 in focal adhesions promotes mechanosensing and functional response in fibroblasts.iScience · 2024Article
- Cellular functions of actin- and microtubule-associated septins.Current biology : CB · 2021Review
- Reconstructed evolutionary history of the yeast septins Cdc11 and Shs1.G3 (Bethesda, Md.) · 2021Article
- Diversity of opisthokont septin proteins reveals structural constraints and conserved motifs.BMC evolutionary biology · 2019Article
- Transfection of choanoflagellates illuminates their cell biology and the ancestry of animal septins.Molecular biology of the cell · 2018Article
- Phosphorylation of Pnut in the Early Stages ofG3 (Bethesda, Md.) · 2018Article
- Article
- Analysis of Septin Reorganization at Cytokinesis Using Polarized Fluorescence Microscopy.Frontiers in cell and developmental biology · 2017Article
- Forging the ring: from fungal septins' divergent roles in morphology, septation and virulence to factors contributing to their assembly into higher order structures.Microbiology (Reading, England) · 2016Review
- Comprehensive Genetic Analysis of Paralogous Terminal Septin Subunits Shs1 and Cdc11 in Saccharomyces cerevisiae.Genetics · 2015Article
- Absolute Arrangement of Subunits in Cytoskeletal Septin Filaments in Cells Measured by Fluorescence Microscopy.Nano letters · 2015Article
- Septins and Generation of Asymmetries in Fungal Cells.Annual review of microbiology · 2015Review
- Septin assemblies form by diffusion-driven annealing on membranes.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Distinct septin heteropolymers co-exist during multicellular development in the filamentous fungus Aspergillus nidulans.PloS one · 2014Article
- Wdpcp, a PCP protein required for ciliogenesis, regulates directional cell migration and cell polarity by direct modulation of the actin cytoskeleton.PLoS biology · 2013Article
- Characterization of TtALV2, an essential charged repeat motif protein of the Tetrahymena thermophila membrane skeleton.Eukaryotic cell · 2013Article
- Filamentous fungal-specific septin AspE is phosphorylated in vivo and interacts with actin, tubulin and other septins in the human pathogen Aspergillus fumigatus.Biochemical and biophysical research communications · 2013Article
- Septin phosphorylation and coiled-coil domains function in cell and septin ring morphology in the filamentous fungus Ashbya gossypii.Eukaryotic cell · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Septins are conserved GTP-binding proteins that assemble into heteromeric complexes that form filaments and higher-order structures in cells. What directs filament assembly, determines the size of higher-order septin structures, and governs septin dynamics is still not well understood. We previously identified two kinases essential for septin ring assembly in the filamentous fungus Ashbya gossypii and demonstrate here that the septin Shs1p is multiphosphorylated at the C-terminus of the protein near the predicted coiled-coil domain. Expression of the nonphosphorylatable allele shs1-9A does not mimic the loss of the kinase nor does complete truncation of the Shs1p C-terminus. Surprisingly, however, loss of the C-terminus or the predicted coiled-coil domain of Shs1p generates expanded zones of septin assemblies and ectopic septin fibers, as well as aberrant cell morphology. The expanded structures form coincident with ring assembly and are heteromeric. Interestingly, while septin recruitment to convex membranes is increased, septin localization is diminished at concave membranes in these mutants. Additionally, the loss of the coiled-coil leads to increased mobility of Shs1p. These data indicate the coiled-coil of Shs1p is an important negative regulator of septin ring size and mobility, and its absence may make septin assembly sensitive to local membrane curvature.
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