Evidence map›Paper›PMID 22767425›Full record

ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2013

Peripheral blood mononuclear cells enhance the anabolic effects of platelet-rich plasma on anterior cruciate ligament fibroblasts.

Ryu Yoshida, Martha M Murray

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed, 1 pooled it
4.1field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Article
  4. Current and Emerging Biologic Therapies for Equine Tendon and Ligament Injuries.The Veterinary clinics of North America. Equine practice · 2025
    Review
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  8. Article
  9. Review
  10. Article
  11. Review
  12. Review
  13. Article
  14. Review
  15. Article
  16. Review
  17. Anterior cruciate ligament repair - past, present and future.Journal of experimental orthopaedics · 2018
    Review
  18. Review
  19. Article
  20. Bench-to-bedside: Bridge-enhanced anterior cruciate ligament repair.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Ryu YoshidaDepartment of Orthopaedic Surgery, Boston Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts 02115, USA.
Martha M Murray
Boston Children's Hospital · US

Funding

The Effect of Age on Functional ACL HealingR01AR054099 · NIAMS · BOSTON CHILDREN'S HOSPITAL · PI MURRAY, MARTHA M · 2006 to 2013
$4.8M
A Novel Mechanism for Intra-articular NonunionR01AR052772 · NIAMS · BOSTON CHILDREN'S HOSPITAL · PI MURRAY, MARTHA M · 2007 to 2010
$1.4M
NIAMS NIH HHS AR054099NIAMS NIH HHS R01 AR052772NIAMS NIH HHS R01 AR054099
6 · The paper itself

Abstract

Use of platelet-rich plasma (PRP) has shown promise in various orthopaedic applications, including treatment of anterior cruciate ligament (ACL) injuries. However, various components of blood, including peripheral blood mononuclear cells (PBMCs), are removed in the process of making PRP. It is yet unknown whether these PBMCs have a positive or negative effect on fibroblast behavior. To begin to define the effect of PBMCs on ACL fibroblasts, ACL fibroblasts were cultured on three-dimensional collagen scaffolds for 14 days with and without PBMCs. ACL fibroblasts exposed to PBMCs showed increased type I and type III procollagen gene expression, collagen protein expression, and cell proliferation when the cells were cultured in the presence of platelets and plasma. However, addition of PBMCs to cells cultured without platelets had no effect. The increase in collagen gene and protein expression was accompanied by an increase in IL-6 expression by the PBMCs with exposure to the platelets. Our results suggest that the interaction between platelets and PBMCs leads to an IL-6 mediated increase in collagen expression by ACL fibroblasts.

Indexed as

AnimalsAnterior Cruciate LigamentAnterior Cruciate Ligament InjuriesCattleCells, CulturedCoculture TechniquesCollagen Type ICollagen Type IIICulture Media, ConditionedFibroblastsGene ExpressionInterleukin-6Knee InjuriesLeukocytes, MononuclearLymphocytesMacrophagesCollagen Type ICollagen Type IIICulture Media, ConditionedInterleukin-6

Identifiers

PMID22767425
PMCPMC3469725
OpenAlexW2032702565

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.