ArticleJournal of environmental monitoring : JEM2012
Utilizing pyrosequencing and quantitative PCR to characterize fungal populations among house dust samples.
Article in Journal of environmental monitoring : JEM, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Microbiota of the indoor environment: a meta-analysis.Microbiome · 2015Pooled it
- Organelle scaling over a 100-fold cell size range.bioRxiv : the preprint server for biology · 2026Article
- Chemical transformation of the multibudding yeast, Aureobasidium pullulans.The Journal of cell biology · 2024Article
- Indoor Bacterial and Fungal Burden in "Moldy" versus "Non-Moldy" Homes: A Case Study Employing Advanced Sequencing Techniques in a US Metropolitan Area.Pathogens (Basel, Switzerland) · 2023Article
- Alteration of oral microbiome composition in children living with pesticide-exposed farm workers.International journal of hygiene and environmental health · 2023Article
- The Mycobiome in Health and Disease: Emerging Concepts, Methodologies and Challenges.Mycopathologia · 2020Review
- Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol.International journal of environmental research and public health · 2020Article
- Current insights into fungal species diversity and perspective on naming the environmental DNA sequences of fungi.Mycology · 2019Review
- A new family and genus inIMA fungus · 2017Article
- Article
- Seasonal and occupational trends of five organophosphate pesticides in house dust.Journal of exposure science & environmental epidemiology · 2017Article
- The roles of the outdoors and occupants in contributing to a potential pan-microbiome of the built environment: a review.Microbiome · 2016Review
- Key determinants of the fungal and bacterial microbiomes in homes.Environmental research · 2015Article
- Article
- Article
- Optimal eukaryotic 18S and universal 16S/18S ribosomal RNA primers and their application in a study of symbiosis.PloS one · 2014Article
- Internal transcribed spacer rRNA gene sequencing analysis of fungal diversity in Kansas City indoor environments.Environmental science. Processes & impacts · 2014Article
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Molecular techniques are an alternative to culturing and counting methods in quantifying indoor fungal contamination. Pyrosequencing offers the possibility of identifying unexpected indoor fungi. In this study, 50 house dust samples were collected from homes in the Yakima Valley, WA. Each sample was analyzed by quantitative PCR (QPCR) for 36 common fungi and by fungal tag-encoded flexible (FLX) amplicon pyrosequencing (fTEFAP) for these and additional fungi. Only 24 of the samples yielded amplified results using fTEFAP but QPCR successfully amplified all 50 samples. Over 450 fungal species were detected by fTEFAP but most were rare. Twenty-two fungi were found by fTEFAP to occur with at least an average of ≥0.5% relative occurrence. Many of these fungi seem to be associated with plants, soil or human skin. Combining fTEFAP and QPCR can enhance studies of fungal contamination in homes.
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Registered trials
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