ArticleThe Journal of surgical research2013
Establishment of a femoral critical-size bone defect model in immunodeficient mice.
Article in The Journal of surgical research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 68 citations in OpenAlex.
- Cerclage Wire as an Affordable Alternative for Internal Fixation in Murine Critical Sized Defect Models.bioRxiv : the preprint server for biology · 2026Article
- Associations between an inflammatory diet index and nonunion: a prospective study of 172,839 UK biobank participants.Frontiers in nutrition · 2025Article
- Temporal dynamics of immune-stromal cell interactions in fracture healing.Frontiers in immunology · 2024Review
- A new method for segmentation and analysis of bone callus in rodent fracture models using micro-CT.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023Article
- Pharmacodynamics and acute toxicity studies of Shangke Jiegu tablet with or without cinnabar.Heliyon · 2022Article
- Differential dynamics of bone graft transplantation and mesenchymal stem cell therapy during bone defect healing in a murine critical size defect.Journal of orthopaedic translation · 2022Article
- Novel Techniques and Future Perspective for Investigating Critical-Size Bone Defects.Bioengineering (Basel, Switzerland) · 2022Review
- Ageing attenuates bone healing by mesenchymal stem cells in a microribbon hydrogel with a murine long bone critical-size defect model.Immunity & ageing : I & A · 2022Article
- Fracture-induced pain-like behaviours in a femoral fracture mouse model.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2021Article
- Treatment of Critical-Size Femoral Bone Defects with Chitosan Scaffolds Produced by a Novel Process from Textile Engineering.Biomedicines · 2021Article
- Chemotactic and Angiogenic Potential of Mineralized Collagen Scaffolds Functionalized with Naturally Occurring Bioactive Factor Mixtures to Stimulate Bone Regeneration.International journal of molecular sciences · 2021Article
- Cell spheroids are as effective as single cells suspensions in the treatment of critical-sized bone defects.BMC musculoskeletal disorders · 2021Article
- Spatio-temporal characterization of fracture healing patterns and assessment of biomaterials by time-lapsed in vivo micro-computed tomography.Scientific reports · 2021Article
- PDGF-BB and IL-4 co-overexpression is a potential strategy to enhance mesenchymal stem cell-based bone regeneration.Stem cell research & therapy · 2021Article
- Sex Differences in Mesenchymal Stem Cell Therapy With Gelatin-Based Microribbon Hydrogels in a Murine Long Bone Critical-Size Defect Model.Frontiers in bioengineering and biotechnology · 2021Article
- Ischial tuberosity: new donor site for bone grafts in animal cleft research.Scientific reports · 2020Article
- Interleukin-4 overexpressing mesenchymal stem cells within gelatin-based microribbon hydrogels enhance bone healing in a murine long bone critical-size defect model.Journal of biomedical materials research. Part A · 2020Article
- Poly(POG)n loaded with recombinant human bone morphogenetic protein-2 accelerates new bone formation in a critical-sized bone defect mouse model.Journal of orthopaedic surgery and research · 2020Article
- Decellularized adipose matrix provides an inductive microenvironment for stem cells in tissue regeneration.World journal of stem cells · 2020Review
- Modeling Complex Orthopedic Trauma in Rodents: Bone, Muscle and Nerve Injury and Healing.Frontiers in pharmacology · 2020Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundThe development of innovative therapies for bone regeneration requires the use of advanced site-specific bone defect small-animal models. The achievement of proper fixation with a murine model is challenging due to the small dimensions of the murine femur. The aim of this investigation was to find the optimal defect size for a murine critical-size bone defect model using external fixation method.
methodsAn external fixation device was attached to the right femur of 30 mice. Femoral bone defects of 1 mm (n = 10), 2 mm (n = 10), and 3 mm (n = 10) were created. Wounds were closed without any additional treatment. To investigate bone healing during the 12-wk observation period, x-ray analysis, histomorphology, immunohistochemistry, and μCT scans were performed.
resultsMicroCT analyses after 12 wk showed that 3/8 1-mm defects, 5/8 2-mm defects, and 8/8 3-mm defects remained as nonunions. The defect volumes were 0.36 ± 0.42 mm³ (1-mm group), 1.40 ± 0.88 mm³ (2-mm group), and 2.88 ± 0.28 mm³ (3-mm group; P < 0.001, between all groups).
conclusionUsing external fixation, a defect size of 3 mm is necessary to reliably create a persisting femoral bone defect in nude mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.