ArticleBiochemistry2012
Using solid-state NMR to monitor the molecular consequences of Cryptococcus neoformans melanization with different catecholamine precursors.
Article in Biochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 51 citations in OpenAlex.
- Microbial melanin: biosynthesis, functional Insights, and emerging research challenges.World journal of microbiology & biotechnology · 2026Review
- Polymorphic α-Glucans as Structural Scaffolds in Cryptococcus Cell Walls for Chitin, Capsule, and Melanin: Insights FromAngewandte Chemie (International ed. in English) · 2025Article
- Identification of widely conserved biosynthetic gene cluster involved in pigment production ofmSystems · 2025Article
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- Melanin in fungi: advances in structure, biosynthesis, regulation, and metabolic engineering.Microbial cell factories · 2024Review
- Molecular architecture of chitin and chitosan-dominated cell walls in zygomycetous fungal pathogens by solid-state NMR.Nature communications · 2024Article
- New Vision of Cell Walls inJournal of fungi (Basel, Switzerland) · 2024Review
- Polysaccharide assemblies in fungal and plant cell walls explored by solid-state NMR.Structure (London, England : 1993) · 2023Review
- Cuticular profiling of insecticide resistant Aedes aegypti.Scientific reports · 2023Article
- Cuticular profiling of insecticide resistantbioRxiv : the preprint server for biology · 2023Article
- Solid-State NMR Investigations of Extracellular Matrixes and Cell Walls of Algae, Bacteria, Fungi, and Plants.Chemical reviews · 2022Review
- Methamphetamine Enhances Cryptococcus neoformans Melanization, Antifungal Resistance, and Pathogenesis in a Murine Model of Drug Administration and Systemic Infection.Infection and immunity · 2022Article
- Cryptococcus neoformans melanization incorporates multiple catecholamines to produce polytypic melanin.The Journal of biological chemistry · 2022Article
- The Role of Melanin in the Biology and Ecology of Nematophagous Fungi.Journal of chemical ecology · 2021Article
- Fungal Melanin and the Mammalian Immune System.Journal of fungi (Basel, Switzerland) · 2021Review
- Unconventional Constituents and Shared Molecular Architecture of the Melanized Cell Wall ofJournal of fungi (Basel, Switzerland) · 2020Article
- Production and characterization of melanin pigments derived from Amorphotheca resinae.Journal of microbiology (Seoul, Korea) · 2020Article
- Melanin deposition in twoThe Journal of biological chemistry · 2020Article
- The structural unit of melanin in the cell wall of the fungal pathogenThe Journal of biological chemistry · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Melanins are a class of natural pigments associated with a wide range of biological functions, including microbial virulence, energy transduction, and protection against solar radiation. Because of their insolubility and structural heterogeneity, solid-state nuclear magnetic resonance (NMR) spectroscopy provides an unprecedented means to define the molecular architecture of these enigmatic pigments. The requirement of obligatory catecholamines for melanization of the pathogenic fungus Cryptococcus neoformans also offers unique opportunities for investigating melanin development. In the current study, pigments produced with L-dopa, methyl-L-dopa, epinephrine, and norepinephrine precursors are compared structurally using (13)C and (1)H magic-angle spinning (MAS) NMR. Striking structural differences were observed for both aromatic and aliphatic molecular constituents of the mature fungal pigment assemblies, thus making it possible to redefine the molecular prerequisites for formation of the aromatic domains of insoluble indole-based biopolymers, to rationalize their distinctive physical characteristics, and to delineate the role of cellular constituents in assembly of the melanized macromolecules with polysaccharides and fatty acyl chain-containing moieties. By achieving an augmented understanding of the mechanisms of C. neoformans melanin biosynthesis and cellular assembly, such studies can guide future drug discovery efforts related to melanin-associated virulence, resistance to tumor therapy, and production of melanin mimetics under cell-free conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.