ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012
Lack of molecular-anatomical evidence for GABAergic influence on axon initial segment of cerebellar Purkinje cells by the pinceau formation.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 58 citations in OpenAlex.
- HCAR1-mediated lactate signaling modulates motor behavior and regulates spontaneous firing in Purkinje cells.iScience · 2026Article
- Structural and functional evidence for ephaptic control of Purkinje cell spike timing by networks of molecular layer interneurons.bioRxiv : the preprint server for biology · 2025Article
- Anti-Kv1.2 Immunoprecipitation Identifies shared mGluR1-Associated Signalosome Complex proteins and PKC-Mediated Regulation of Kv1.2 in the Cerebellum.bioRxiv : the preprint server for biology · 2025Article
- Article
- Axon initial segment structure and function in health and disease.Physiological reviews · 2025Review
- Absolute Number of Three Populations of Interneurons and All GABAergic Synapses in the Human Hippocampus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- The role of astrocytes from synaptic to non-synaptic plasticity.Frontiers in cellular neuroscience · 2024Review
- Glyoxal fixation: An approach to solve immunohistochemical problem in neuroscience research.Science advances · 2023Article
- Basket to Purkinje Cell Inhibitory Ephaptic Coupling Is Abolished in Episodic Ataxia Type 1.Cells · 2023Article
- The Cerebellar Cortex.Annual review of neuroscience · 2022Review
- Perisomatic innervation and neurochemical features of giant pyramidal neurons in both hemispheres of the human primary motor cortex.Brain structure & function · 2021Article
- Article
- Dystroglycan Mediates Clustering of Essential GABAergic Components in Cerebellar Purkinje Cells.Frontiers in molecular neuroscience · 2020Article
- Cellular and Subcellular Localization of Endogenous Neuroligin-1 in the Cerebellum.Cerebellum (London, England) · 2018Article
- Mutant ataxin1 disrupts cerebellar development in spinocerebellar ataxia type 1.The Journal of clinical investigation · 2018Article
- Elavl3 is essential for the maintenance of Purkinje neuron axons.Scientific reports · 2018Article
- Synaptic Activation of a Detailed Purkinje Cell Model Predicts Voltage-Dependent Control of Burst-Pause Responses in Active Dendrites.Frontiers in cellular neuroscience · 2017Article
- Cooperative Interactions between 480 kDa Ankyrin-G and EB Proteins Assemble the Axon Initial Segment.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Dopamine synapse is a neuroligin-2-mediated contact between dopaminergic presynaptic and GABAergic postsynaptic structures.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Molecular layer interneurons of the cerebellum: developmental and morphological aspects.Cerebellum (London, England) · 2015Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The axon initial segment (AIS) of cerebellar Purkinje cells (PCs) is embraced by ramified axons of GABAergic basket cells (BCs) called the pinceau formation. This unique structure has been assumed to be a device for the modulation of PC outputs through electrical and/or GABAergic inhibition. Electrical inhibition is supported by enriched potassium channels, absence of sodium channels, and developed septate-like junctions between BC axons. The neurochemical basis for GABAergic inhibition, however, has not been well investigated. Here we addressed this issue using C56BL/6 mice. First, we confirmed previous observations that typical synaptic contacts were rare and confined to proximal axonal portions, with the remaining portions being mostly covered by astrocytic processes. Then we examined the expression of molecules involved in GABAergic signaling, including GABA synthetic enzyme glutamic acid decarboxylase (GAD), vesicular GABA transporter vesicular inhibitory amino acid transporter (VIAAT), cytomatrix active zone protein bassoon, GABA receptor GABA(A)Rα1, and cell adhesion molecule neuroligin-2. These molecules were recruited to form a functional assembly at perisomatic BC-PC synapses and along the AIS of hippocampal and neocortical pyramidal cells. GAD and VIAAT immunogold labeling was five times lower in the pinceau formation compared with perisomatic BC terminals and showed no accumulation toward the AIS. Moreover, bassoon, neuroligin-2, and GABA(A)Rα1 formed no detectable clusters along the ankyrin-G-positive AIS proper. These findings indicate that GABAergic signaling machinery is organized loosely and even incompletely in the pinceau formation. Together, BCs do not appear to exert GABAergic synaptic inhibition on the AIS, although the mode of action of the pinceau formation remains to be explored.
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