ArticleMolecular biology reports2012
Identification and characterization of microRNA in the dairy goat (Capra hircus) mammary gland by Solexa deep-sequencing technology.
Article in Molecular biology reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 89 citations in OpenAlex.
- Transcriptomic analysis of mammary gland tissues in lactating and non-lactating dairy goats reveals miRNA-mediated regulation of lactation, involution, and remodeling.Frontiers in cell and developmental biology · 2025Article
- Hormonal regulation of miRNA during mammary gland development.Biology open · 2024Article
- miR-497 RegulatesGenes · 2023Article
- MicroRNAs in Ruminants and Their Potential Role in Nutrition and Physiology.Veterinary sciences · 2023Review
- Identification and Characterization of MicroRNAs Involving in Initial Sex Differentiation ofBiology · 2022Article
- The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep.Non-coding RNA · 2021Review
- Biological Characteristics and Roles of Noncoding RNAs in Milk-Derived Extracellular Vesicles.Advances in nutrition (Bethesda, Md.) · 2021Review
- Review
- Differentially Expressed MiRNAs of Goat Submandibular Glands Among Three Developmental Stages Are Involved in Immune Functions.Frontiers in genetics · 2021Article
- Identification and expression profile of microRNA in seven tissues of the Golden snub-nosed monkey (Rhinopithecus roxellanae).Molecular genetics and genomics : MGG · 2020Article
- Differential expression of novel MicroRNAs from developing fetal heart of Gallus gallus domesticus implies a role in cardiac development.Molecular and cellular biochemistry · 2019Article
- Comparative Identification of MicroRNAs inInsects · 2019Article
- Deep RNA-Seq reveals miRNome differences in mammary tissue of lactating Holstein and Montbéliarde cows.BMC genomics · 2019Article
- Selection signature analysis reveals genes underlying sheep milking performance.Archives animal breeding · 2019Article
- Small RNA-Seq reveals novel miRNAs shaping the transcriptomic identity of rat brain structures.Life science alliance · 2018Article
- Article
- Screening of miRNA profiles and construction of regulation networks in early and late lactation of dairy goat mammary glands.Scientific reports · 2017Article
- Article
- Identification and profiling of Cyprinus carpio microRNAs during ovary differentiation by deep sequencing.BMC genomics · 2017Article
- The tammar wallaby: A marsupial model to examine the timed delivery and role of bioactives in milk.General and comparative endocrinology · 2017Review
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
microRNAs (miRNAs) perform critical roles in various biological and metabolic processes by regulating gene expression at the post-transcriptional level. To investigate the functional roles of miRNAs in the lactating mammary gland of Capra hircus, a library was constructed from the lactating mammary glands of Laoshan dairy goats (C. hircus) during early lactation. The miRNA expression profiles were systematically screened, and miRNAs were identified and characterized using Solexa deep-sequencing technology and bioinformatics. As a result, a total of 18,031,615 clean reads were obtained representing 305,711 unique sRNAs. A total of 12,086,616 sRNAs representing 3,701 unique sRNAs matched the known Bos taurus miRNA precursors in miRBase 17.0, and 300 known miRNAs and 15 miRNA were discovered. In addition, 131 novel miRNAs sequences were also obtained, and 147,703 putative targets were predicted. GO and KEGG pathway analysis showed that the majority of targets were involved in cellular processes and metabolic pathways. The 290 known miRNAs, 14 miRNA and 38 novel miRNAs were validated by sequencing a second library that was constructed from the same tissues as the first library. Our study provided the first large-scale identification and characterization of miRNAs in the mammary gland tissue of the dairy goat. The results indicate that the regulation of miRNA-mediated gene expression occurs during early lactation in dairy goats. This study significantly enriches the C. hircus miRNA repertoire and provides a reference for the elucidation of complex miRNA-mediated regulatory networks for gene expression in the physiology and developmental progression of the lactating mammary gland.
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