ArticleThe ISME journal2012
Nitrification expanded: discovery, physiology and genomics of a nitrite-oxidizing bacterium from the phylum Chloroflexi.
Article in The ISME journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
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Who cites it
108 citing papers in PubMed.
- Integrated omics analyses reveal insights into microbial dark matter associated with deep-sea water in Central Indian Ocean.World journal of microbiology & biotechnology · 2026Article
- The Microbiome of an Invasive Antarctic insect, Eretmoptera Murphyi (Diptera: Chironomidae), and its Potential Role in Nutrient Cycling.Microbial ecology · 2026Article
- Kinetic plasticity of nitrite-oxidizing bacteria containing cytoplasmic nitrite oxidoreductase.The ISME journal · 2026Article
- Elevated temperature simulating heatwaves restructures active nitrifying communities and associated viruses in tidal flats and agricultural soils.The ISME journal · 2026Article
- Chloroflexota in agricultural soils: current knowledge and future research directions.Frontiers in microbiology · 2026Review
- Genomics and physiological characterizations of an acidotolerant nitrite-oxidizingApplied and environmental microbiology · 2025Article
- Contrasting diversity and temporal patterns in leaf and root microbiome of two nearby temperate Zostera marina meadows.Environmental microbiome · 2025Article
- Biogeographic Patterns and Ecological Roles of Microorganisms in Sediments Along an Estuarine Salinity Gradient.Environmental microbiology reports · 2025Article
- Advanced Technologies for Nitrogen Removal and Recovery from Municipal and Industrial Wastewater.Materials (Basel, Switzerland) · 2025Review
- Spatial distribution and source apportionment of nitrogen in typical plain river networks and bacterial community response.Frontiers in microbiology · 2025Article
- Physiological and Genomic Characterization of Oligotrophic Nitrobacter Isolated from a Forest Soil in Japan.Microbes and environments · 2025Article
- Hyperexpansion of genetic diversity and metabolic capacity of extremophilic bacteria and archaea in ancient Andean lake sediments.Microbiome · 2024Article
- Physiological adaptation and population dynamics of a nitrifying sludge exposed to ampicillin.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Article
- An abundant bacterial phylum with nitrite-oxidizing potential in oligotrophic marine sediments.Communications biology · 2024Article
- Deep-seamBio · 2024Article
- Biofilm colonization and succession in a full-scale partial nitritation-anammox moving bed biofilm reactor.Microbiome · 2024Article
- Dynamics of Methane-Consuming Biomes from Wieliczka Formation: Environmental and Enrichment Studies.Biology · 2023Article
- Cultivation and genomic characterization of novel and ubiquitous marine nitrite-oxidizing bacteria from the Nitrospirales.The ISME journal · 2023Article
- Characterisation of bacteria representing a novel Nitrosomonas clade: Physiology, genomics and distribution of missing ammonia oxidizer.Environmental microbiology reports · 2023Article
48 more citing papers are in PubMed but not listed here.
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12 authors.
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Abstract
Nitrite-oxidizing bacteria (NOB) catalyze the second step of nitrification, a major process of the biogeochemical nitrogen cycle, but the recognized diversity of this guild is surprisingly low and only two bacterial phyla contain known NOB. Here, we report on the discovery of a chemolithoautotrophic nitrite oxidizer that belongs to the widespread phylum Chloroflexi not previously known to contain any nitrifying organism. This organism, named Nitrolancetus hollandicus, was isolated from a nitrifying reactor. Its tolerance to a broad temperature range (25-63 °C) and low affinity for nitrite (K(s)=1 mM), a complex layered cell envelope that stains Gram positive, and uncommon membrane lipids composed of 1,2-diols distinguish N. hollandicus from all other known nitrite oxidizers. N. hollandicus grows on nitrite and CO(2), and is able to use formate as a source of energy and carbon. Genome sequencing and analysis of N. hollandicus revealed the presence of all genes required for CO(2) fixation by the Calvin cycle and a nitrite oxidoreductase (NXR) similar to the NXR forms of the proteobacterial nitrite oxidizers, Nitrobacter and Nitrococcus. Comparative genomic analysis of the nxr loci unexpectedly indicated functionally important lateral gene transfer events between Nitrolancetus and other NOB carrying a cytoplasmic NXR, suggesting that horizontal transfer of the NXR module was a major driver for the spread of the capability to gain energy from nitrite oxidation during bacterial evolution. The surprising discovery of N. hollandicus significantly extends the known diversity of nitrifying organisms and likely will have implications for future research on nitrification in natural and engineered ecosystems.
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