ArticleMolecular biotechnology2013
Pathogenesis-related gene, JcPR-10a from Jatropha curcas exhibit RNase and antifungal activity.
Article in Molecular biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 2 of them syntheses that pooled it.
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Who cites it
18 citing papers in PubMed, 2 syntheses or guidelines pooled it, 48 citations in OpenAlex.
- Pathogenesis-related protein 10 in resistance to biotic stress: progress in elucidating functions, regulation and modes of action.Frontiers in plant science · 2023Pooled it
- Genetic Improvement for Resistance to Black Sigatoka in Bananas: A Systematic Review.Frontiers in plant science · 2021Pooled it
- Heterologous expression of Halostachys caspica pathogenesis-related protein 10 increases salt and drought resistance in transgenic Arabidopsis thaliana.Plant molecular biology · 2024Article
- Major latex-like proteins show pH dependency in their binding to hydrophobic organic pollutants.Journal of pesticide science · 2023Article
- Letter to the Editor: Cautionary Note on Ribonuclease Activity of Recombinant PR-10 Proteins.Plant & cell physiology · 2023Article
- Decrypting the multi-functional biological activators and inducers of defense responses against biotic stresses in plants.Heliyon · 2023Review
- The AP2/ERF GmERF113 Positively Regulates the Drought Response by ActivatingInternational journal of molecular sciences · 2022Article
- Gene Co-expression Network Analysis of the Comparative Transcriptome Identifies Hub Genes Associated With Resistance toFrontiers in plant science · 2022Article
- A Pathogenesis-Related Protein-Like Gene Is Involved in theFrontiers in plant science · 2020Article
- Article
- A sugarcane pathogenesis-related protein, ScPR10, plays a positive role in defense responses under Sporisorium scitamineum, SrMV, SA, and MeJA stresses.Plant cell reports · 2017Article
- A pathogenesis related-10 protein CaARP functions as aldo/keto reductase to scavenge cytotoxic aldehydes.Plant molecular biology · 2016Article
- Improved Shoot Regeneration, Salinity Tolerance and Reduced Fungal Susceptibility in Transgenic Tobacco Constitutively Expressing PR-10a Gene.Frontiers in plant science · 2016Article
- Article
- Molecular characteristics and biochemical functions of VpPR10s from Vitis pseudoreticulata associated with biotic and abiotic stresses.International journal of molecular sciences · 2014Article
- Molecular cloning and characterization of a group II WRKY transcription factor from Jatropha curcas, an important biofuel crop.DNA and cell biology · 2014Article
- The pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca(2+) and Mg(2+) dependent-DNase activity and antifungal action on Moniliophthora perniciosa.BMC plant biology · 2014Article
- Pathogenesis related-10 proteins are small, structurally similar but with diverse role in stress signaling.Molecular biology reports · 2014Review
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pathogenesis-related proteins have a broad spectrum of roles, ranging from seed germination, development to resistance. The PR-10 is a multigene family differing from other PR proteins in being intracellular, small and acidic with similar 3D structures. We have isolated JcPR-10a cDNA with an ORF of 483 bp from J. curcas, an important biofuel crop grown in the wastelands of India. JcPR-10a gets clustered with dicots in phylogenetic tree. The genomic organisation analysis of JcPR-10a revealed the presence of an intron at conserved 185 bp position. Transcript expression of JcPR-10a was upregulated in response to different stimuli such as NaCl, salicylic acid, methyl jasmonate and M. phaseolina. In response to SA and Macrophomina the transcript was found increased at 48 h, however, in case of NaCl and MeJa a strong induction was observed at 12 h which decreased at 48 h. We first time report the transcript up regulation of PR-10 gene by Macrophomina, a pathogen causing collar rot in Jatropha. The recombinant E. coli cells showed better growth in LB medium supplemented with NaCl, whereas growth of recombinant cells was inhibited in LB medium supplemented with KCl, mannitol, sorbitol, methyl jasmonate and salicylic acid. The JcPR-10a protein was overexpressed in E. coli cells, and was purified to homogeneity, the purified protein exhibited RNase and DNase activity. Furthermore, the protein also showed antifungal activity against Macrophomina, indicating that JcPR-10a can serve as an important candidate to engineer stress tolerance in Jatropha as well as other plants susceptible to collar rot by Macrophomina.
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