ArticleEuropean journal of human genetics : EJHG2013
Variable imprinting of the MEST gene in human preimplantation embryos.
Article in European journal of human genetics : EJHG, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Association Between Human Embryo Culture Conditions, Cryopreservation, and the Potential Risk of Birth Defects in Children Conceived Through Assisted Reproduction Technology.Medicina (Kaunas, Lithuania) · 2025Review
- Marsupials have monoallelic MEST expression with a conserved antisense lncRNA but MEST is not imprinted.Heredity · 2024Article
- Ectoine Globally Hypomethylates DNA in Skin Cells and Suppresses Cancer Proliferation.Marine drugs · 2023Article
- Inference of putative cell-type-specific imprinted regulatory elements and genes during human neuronal differentiation.Human molecular genetics · 2023Article
- Embryo cryopreservation leads to sex-specific DNA methylation perturbations in both human and mouse placentas.Human molecular genetics · 2022Article
- Clomifene and Assisted Reproductive Technology in Humans Are Associated with Sex-Specific Offspring Epigenetic Alterations in Imprinted Control Regions.International journal of molecular sciences · 2022Article
- Epigenetic Risks of Medically Assisted Reproduction.Journal of clinical medicine · 2022Review
- Contemporary Use of ICSI and Epigenetic Risks to Future Generations.Journal of clinical medicine · 2022Review
- Effects of Cryopreservation on Sperm with Cryodiluent in Viviparous Black Rockfish (International journal of molecular sciences · 2022Article
- Genetic variation in placental insufficiency: What have we learned over time?Frontiers in cell and developmental biology · 2022Review
- Long intergenic noncoding RNA LINC00284 knockdown reduces angiogenesis in ovarian cancer cellsFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Allelic Switching ofInternational journal of genomics · 2019Article
- Stressful Newborn Memories: Pre-Conceptual,Frontiers in psychiatry · 2019Review
- Placental imprinting variation associated with assisted reproductive technologies and subfertility.Epigenetics · 2017Article
- Systemic analysis of osteoblast-specific DNA methylation marks reveals novel epigenetic basis of osteoblast differentiation.Bone reports · 2017Article
- Isolation and expression of the human gametocyte-specific factor 1 gene (GTSF1) in fetal ovary, oocytes, and preimplantation embryos.Journal of assisted reproduction and genetics · 2017Article
- Human Oocyte-Derived Methylation Differences Persist in the Placenta Revealing Widespread Transient Imprinting.PLoS genetics · 2016Article
- Epigenetics: A key paradigm in reproductive health.Clinical and experimental reproductive medicine · 2016Review
- Imprinted gene expression in hybrids: perturbed mechanisms and evolutionary implications.Heredity · 2014Review
- Quantitative expression of developmental genes, Pou5f1 (Oct4) and Mest (Peg1), in vitrified mouse embryos.Iranian journal of reproductive medicine · 2013Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
There is evidence that expression and methylation of the imprinted paternally expressed gene 1/mesoderm-specific transcript homologue (PEG1/MEST) gene may be affected by assisted reproductive technologies (ARTs) and infertility. In this study, we sought to assess the imprinting status of the MEST gene in a large cohort of in vitro-derived human preimplantation embryos, in order to characterise potentially adverse effects of ART and infertility on this locus in early human development. Embryonic genomic DNA from morula or blastocyst stage embryos was screened for a transcribed AflIII polymorphism in MEST and imprinting analysis was then performed in cDNA libraries derived from these embryos. In 10 heterozygous embryos, MEST expression was monoallelic in seven embryos, predominantly monoallelic in two embryos, and biallelic in one embryo. Screening of cDNA derived from 61 additional human preimplantation embryos, for which DNA for genotyping was unavailable, identified eight embryos with expression originating from both alleles (biallelic or predominantly monoallelic). In some embryos, therefore, the onset of imprinted MEST expression occurs during late preimplantation development. Variability in MEST imprinting was observed in both in vitro fertilization and intracytoplasmic sperm injection-derived embryos. Biallelic or predominantly monoallelic MEST expression was not associated with any one cause of infertility. Characterisation of the main MEST isoforms revealed that isoform 2 was detected in early development and was itself variably imprinted between embryos. To our knowledge, this report constitutes the largest expression study to date of genomic imprinting in human preimplantation embryos and reveals that for some imprinted genes, contrasting imprinting states exist between embryos.
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