ArticleScience signaling2012
Quantitative imaging of epithelial cell scattering identifies specific inhibitors of cell motility and cell-cell dissociation.
Article in Science signaling, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Topological data analysis of collective and individual epithelial cells using persistent homology of loops.Soft matter · 2021Article
- The epithelial-mesenchymal transition and the cytoskeleton in bioengineered systems.Cell communication and signaling : CCS · 2021Review
- Reoccurring neural stem cell divisions in the adult zebrafish telencephalon are sufficient for the emergence of aggregated spatiotemporal patterns.PLoS biology · 2020Article
- Junction Mapper is a novel computer vision tool to decipher cell-cell contact phenotypes.eLife · 2019Article
- Motility-limited aggregation of mammary epithelial cells into fractal-like clusters.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Src- and confinement-dependent FAK activation causes E-cadherin relaxation and β-catenin activity.The Journal of cell biology · 2018Article
- Homogenizing cellular tension by hepatocyte growth factor in expanding epithelial monolayer.Scientific reports · 2017Article
- Defining functional interactions during biogenesis of epithelial junctions.Nature communications · 2016Article
- Article
- VASP, zyxin and TES are tension-dependent members of Focal Adherens Junctions independent of the α-catenin-vinculin module.Scientific reports · 2015Article
- The motility-proliferation-metabolism interplay during metastatic invasion.Scientific reports · 2015Article
- Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- Cyclooxygenase 1 mRNA expression is undetectable in Madin Darby Canine Kidney cells.BMC research notes · 2015Article
- Basonuclin-1 modulates epithelial plasticity and TGF-β1-induced loss of epithelial cell integrity.Oncogene · 2015Article
- Protrusive activity guides changes in cell-cell tension during epithelial cell scattering.Biophysical journal · 2014Article
- Propagating waves of directionality and coordination orchestrate collective cell migration.PLoS computational biology · 2014Article
- Cadherin mechanotransduction in tissue remodeling.Cellular and molecular life sciences : CMLS · 2013Review
- HGF signaling regulates Claudin-3 dynamics through its C-terminal tyrosine residues.Tissue barriers · 2013Article
- Loss of integrin α3 prevents skin tumor formation by promoting epidermal turnover and depletion of slow-cycling cells.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Vinculin-dependent Cadherin mechanosensing regulates efficient epithelial barrier formation.Biology open · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The scattering of cultured epithelial cells in response to hepatocyte growth factor (HGF) is a model system that recapitulates key features of metastatic cell behavior in vitro, including disruption of cell-cell adhesions and induction of cell migration. We have developed image analysis tools that do not require fluorescence tagging and that automatically track and characterize three aspects of scattering in live cells: increase in cell motility, loss of cell-cell adhesion, and spatial dispersion of cells (the redistribution of cells during scattering). We used these tools to screen a library of drugs, and we identified several efficient inhibitors of scattering, which we classified as selective inhibitors of either motility or loss of cell-cell adhesion, or as nonselective inhibitors. We validated the inhibitors and putative targets from this screen in two unrelated model cell lines. Using pharmacological treatments and RNA interference (RNAi), we found that nonsteroidal anti-inflammatory drugs inhibited cell-cell dissociation, that indirubins inhibited cell motility, and that cyclin-dependent kinase 1 and ribosomal S6 kinase were signaling intermediates in HGF-induced cell scattering. This assay is suitable for larger-scale screenings of chemical compounds or RNAi libraries.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.