ArticlePloS one2012
IL-4 engagement of the type I IL-4 receptor complex enhances mouse eosinophil migration to eotaxin-1 in vitro.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Association of the interleukin-4Rα rs1801275 and rs1805015 polymorphisms with glioma risk.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Pooled it
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- Exploration of miRNAs promoting eosinophilia and type 2 immunity.Frontiers in allergy · 2026Article
- Lymphocytes and monocytes undergo swift suppression of IL-10R, IL-6R, and IL-2Rβγ signaling under high concentrations of different cytokines.bioRxiv : the preprint server for biology · 2025Article
- Linking adipose tissue eosinophils, IL-4, and leptin in human obesity and insulin resistance.JCI insight · 2024Article
- Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.The Journal of allergy and clinical immunology · 2023Article
- Breaking down the complex pathophysiology of eosinophilic esophagitis.Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology · 2023Review
- Eosinophilic esophagitis: Immune mechanisms and therapeutic targets.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2022Review
- Analysis of the signal cross talk via CCL26 in the tumor microenvironment in osteosarcoma.Scientific reports · 2021Article
- The malignancy of liver cancer cells is increased by IL-4/ERK/AKT signaling axis activity triggered by irradiated endothelial cells.Journal of radiation research · 2020Article
- Sex differences in IL-17 contribute to chronicity in male versus female urinary tract infection.JCI insight · 2019Article
- TACI-Deficient Macrophages Protect Mice Against Metaflammation and Obesity-Induced Dysregulation of Glucose Homeostasis.Diabetes · 2018Article
- IL-4 and IL-13 Receptor Signaling From 4PS to Insulin Receptor Substrate 2: There and Back Again, a Historical View.Frontiers in immunology · 2018Review
- Eosinophil persistence in vivo and sustained viability ex vivo in response to respiratory challenge with fungal allergens.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2018Article
- Regulation of Eosinophil and Group 2 Innate Lymphoid Cell Trafficking in Asthma.Frontiers in medicine · 2017Article
- Polymorphism in the IL4R gene and clinical features are associated with glioma prognosis: Analyses of case-cohort studies.Medicine · 2016Observational
- Eosinophils and IL-4 Support Nematode Growth Coincident with an Innate Response to Tissue Injury.PLoS pathogens · 2015Article
- IL-4 and IL-13 signaling in allergic airway disease.Cytokine · 2015Review
- Commentary: IL-4 and IL-13 receptors and signaling.Cytokine · 2015Article
- Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundPrevious work from our laboratory demonstrated that IL-4Rα expression on a myeloid cell type was responsible for enhancement of Th2-driven eosinophilic inflammation in a mouse model of allergic lung inflammation. Subsequently, we have shown that IL-4 signaling through type I IL-4 receptors on monocytes/macrophages strongly induced activation of the IRS-2 pathway and a subset of genes characteristic of alternatively activated macrophages. The direct effect(s) of IL-4 and IL-13 on mouse eosinophils are not clear. The goal of this study was determine the effect of IL-4 and IL-13 on mouse eosinophil function.
methodsStandard Transwell chemotaxis assay was used to assay migration of mouse eosinophils and signal transduction was assessed by Western blotting.
resultsHere we determined that (i) mouse eosinophils express both type I and type II IL-4 receptors, (ii) in contrast to human eosinophils, mouse eosinophils do not chemotax to IL-4 or IL-13 although (iii) pre-treatment with IL-4 but not IL-13 enhanced migration to eotaxin-1. This IL-4-mediated enhancement was dependent on type I IL-4 receptor expression: γC-deficient eosinophils did not show enhancement of migratory capacity when pre-treated with IL-4. In addition, mouse eosinophils responded to IL-4 with the robust tyrosine phosphorylation of STAT6 and IRS-2, while IL-13-induced responses were considerably weaker.
conclusionsThe presence of IL-4 in combination with eotaxin-1 in the allergic inflammatory milieu could potentiate infiltration of eosinophils into the lungs. Therapies that block IL-4 and chemokine receptors on eosinophils might be more effective clinically in reducing eosinophilic lung inflammation.
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