ArticlePloS one2012
Transcriptome analysis during human trophectoderm specification suggests new roles of metabolic and epigenetic genes.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Differences in CXCR4 expression between day 5 and day 6 euploid blastocysts: A predictor of implantation potential in single embryo transfer.Clinical and experimental reproductive medicine · 2026Article
- Gata3 dosage governs primitive endoderm versus trophectoderm specification in embryonic stem cells.EMBO reports · 2026Article
- The GATA3-RFPL3-ASK1 axis suppresses breast cancer growth and lung metastasis.Apoptosis : an international journal on programmed cell death · 2026Article
- Genomics of pregnancy loss.Journal of assisted reproduction and genetics · 2026Review
- Trophoblast adaptation to hypoxia: balance and dysfunction.Cell communication and signaling : CCS · 2025Review
- Photo-click hydrogels for 3D in situ differentiation of pancreatic progenitors from induced pluripotent stem cells.Stem cell research & therapy · 2023Article
- A Kaleidoscope of Keratin Gene Expression and the Mosaic of Its Regulatory Mechanisms.International journal of molecular sciences · 2023Review
- Overview of junctional complexes during mammalian early embryonic development.Frontiers in endocrinology · 2023Review
- Hypothesis: human trophectoderm biopsy downregulates the expression of the placental growth factor gene.Journal of assisted reproduction and genetics · 2021Article
- Short-term Western-style diet negatively impacts reproductive outcomes in primates.JCI insight · 2021Article
- A DNA methylation state transition model reveals the programmed epigenetic heterogeneity in human pre-implantation embryos.Genome biology · 2020Article
- The unknown human trophectoderm: implication for biopsy at the blastocyst stage.Journal of assisted reproduction and genetics · 2020Review
- Importin 13 promotes NSCLC progression by mediating RFPL3 nuclear translocation and hTERT expression upregulation.Cell death & disease · 2020Article
- Can trophectoderm RNA analysis predict human blastocyst competency?Systems biology in reproductive medicine · 2019Article
- Expression Patterns of Circular RNAs in High Quality and Poor Quality Human Spermatozoa.Frontiers in endocrinology · 2019Article
- Exposure to maternal obesity alters gene expression in the preimplantation ovine conceptus.BMC genomics · 2018Article
- Preliminary RNA-Seq Analysis of Long Non-Coding RNAs Expressed in Human Term Placenta.International journal of molecular sciences · 2018Article
- Global, Survival, and Apoptotic Transcriptome during Mouse and Human Early Embryonic Development.BioMed research international · 2018Article
- MouseEvoDevo · 2018Article
- Genetic redundancy of GATA factors in the extraembryonic trophoblast lineage ensures the progression of preimplantation and postimplantation mammalian development.Development (Cambridge, England) · 2017Article
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7 authors.
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Abstract
In humans, successful pregnancy depends on a cascade of dynamic events during early embryonic development. Unfortunately, molecular data on these critical events is scarce. To improve our understanding of the molecular mechanisms that govern the specification/development of the trophoblast cell lineage, the transcriptome of human trophectoderm (TE) cells from day 5 blastocysts was compared to that of single day 3 embryos from our in vitro fertilization program by using Human Genome U133 Plus 2.0 microarrays. Some of the microarray data were validated by quantitative RT-PCR. The TE molecular signature included 2,196 transcripts, among which were genes already known to be TE-specific (GATA2, GATA3 and GCM1) but also genes involved in trophoblast invasion (MUC15), chromatin remodeling (specifically the DNA methyltransferase DNMT3L) and steroid metabolism (HSD3B1, HSD17B1 and FDX1). In day 3 human embryos 1,714 transcripts were specifically up-regulated. Besides stemness genes such as NANOG and DPPA2, this signature included genes belonging to the NLR family (NALP4, 5, 9, 11 and 13), Ret finger protein-like family (RFPL1, 2 and 3), Melanoma Antigen family (MAGEA1, 2, 3, 5, 6 and 12) and previously unreported transcripts, such as MBD3L2 and ZSCAN4. This study provides a comprehensive outlook of the genes that are expressed during the initial embryo-trophectoderm transition in humans. Further understanding of the biological functions of the key genes involved in steroidogenesis and epigenetic regulation of transcription that are up-regulated in TE cells may clarify their contribution to TE specification and might also provide new biomarkers for the selection of viable and competent blastocysts.
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