ArticleThe Journal of endocrinology2012
Duplicated zebrafish co-orthologs of parathyroid hormone-related peptide (PTHrP, Pthlh) play different roles in craniofacial skeletogenesis.
Article in The Journal of endocrinology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Connecting Bone Remodeling and Regeneration: Unraveling Hormones and Signaling Pathways.Biology · 2025Review
- Structure and function of the larval teleost fish gill.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2024Review
- Antagonistic interaction between caffeine and ketamine in zebrafish: Implications for aquatic toxicity.Environmental science and ecotechnology · 2024Article
- Probiotics Enhance Bone Growth and Rescue BMP Inhibition: New Transgenic Zebrafish Lines to Study Bone Health.International journal of molecular sciences · 2022Article
- Pthlha and mechanical force control early patterning of growth zones in the zebrafish craniofacial skeleton.Development (Cambridge, England) · 2022Article
- The Signalling Effects of Photobiomodulation on Osteoblast Proliferation, Maturation and Differentiation: A Review.Stem cell reviews and reports · 2021Review
- Type II NaPflugers Archiv : European journal of physiology · 2019Review
- Zebrafish as an Emerging Model for Osteoporosis: A Primary Testing Platform for Screening New Osteo-Active Compounds.Frontiers in endocrinology · 2019Review
- Stress, Glucocorticoids and Bone: A Review From Mammals and Fish.Frontiers in endocrinology · 2018Review
- Pth4, an ancient parathyroid hormone lost in eutherian mammals, reveals a new brain-to-bone signaling pathway.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Article
- PTH Reloaded: A New Evolutionary Perspective.Frontiers in physiology · 2017Review
- Identification of prohormones and pituitary neuropeptides in the African cichlid, Astatotilapia burtoni.BMC genomics · 2016Article
- Bone-remodeling transcript levels are independent of perching in end-of-lay white leghorn chickens.International journal of molecular sciences · 2015Article
- Genome-Wide Identification of Calcium Dependent Protein Kinase Gene Family in Plant Lineage Shows Presence of Novel D-x-D and D-E-L Motifs in EF-Hand Domain.Frontiers in plant science · 2015Article
- Zebrafish Craniofacial Development: A Window into Early Patterning.Current topics in developmental biology · 2015Review
- Zebrafish Bone and General Physiology Are Differently Affected by Hormones or Changes in Gravity.PloS one · 2015Article
- Inhibitory function of parathyroid hormone-related protein on chondrocyte hypertrophy: the implication for articular cartilage repair.Arthritis research & therapy · 2012Review
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
In mammals, parathyroid hormone-related peptide (PTHrP, alias PTH-like hormone (Pthlh)) acts as a paracrine hormone that regulates the patterning of cartilage, bone, teeth, pancreas, and thymus. Beyond mammals, however, little is known about the molecular genetic mechanisms by which Pthlh regulates early development. To evaluate conserved pathways of craniofacial skeletogenesis, we isolated two Pthlh co-orthologs from the zebrafish (Danio rerio) and investigated their structural, phylogenetic, and syntenic relationships, expression, and function. Results showed that pthlh duplicates originated in the teleost genome duplication. Zebrafish pthlha and pthlhb were maternally expressed and showed overlapping and distinct zygotic expression patterns during skeletal development that mirrored mammalian expression domains. To explore the regulation of duplicated pthlh genes, we studied their expression patterns in mutants and found that both sox9a and sox9b are upstream of pthlha in arch and fin bud cartilages, but only sox9b is upstream of pthlha in the pancreas. Morpholino antisense knockdown showed that pthlha regulates both sox9a and sox9b in the pharyngeal arches but not in the brain or otic vesicles and that pthlhb does not regulate either sox9 gene, which is likely related to its highly degraded nuclear localization signal. Knockdown of pthlha but not pthlhb caused runx2b overexpression in craniofacial cartilages and premature bone mineralization. We conclude that in normal cartilage development, sox9 upregulates pthlh, which downregulates runx2, and that the duplicated nature of all three of these genes in zebrafish creates a network of regulation by different co-orthologs in different tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.