ArticleThe Journal of membrane biology2012
Connexin composition in apposed gap junction hemiplaques revealed by matched double-replica freeze-fracture replica immunogold labeling.
Article in The Journal of membrane biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Gap junction intercellular communications regulates activation of SARM1 and protects against axonal degeneration.Cell death & disease · 2025Article
- GAP junctions: multifaceted regulators of neuronal differentiation.Tissue barriers · 2022Article
- Effects of Constitutive and Acute Connexin 36 Deficiency on Brain-Wide Susceptibility to PTZ-Induced Neuronal Hyperactivity.Frontiers in molecular neuroscience · 2020Article
- Understanding the Molecular and Cell Biological Mechanisms of Electrical Synapse Formation.Frontiers in neuroanatomy · 2020Article
- Connexin30 and Connexin43 show a time-of-day dependent expression in the mouse suprachiasmatic nucleus and modulate rhythmic locomotor activity in the context of chronodisruption.Cell communication and signaling : CCS · 2019Article
- Connexins and Disease.Cold Spring Harbor perspectives in biology · 2018Review
- Freeze fracture: new avenues for the ultrastructural analysis of cells in vitro.Histochemistry and cell biology · 2018Review
- Connexins in Cardiovascular and Neurovascular Health and Disease: Pharmacological Implications.Pharmacological reviews · 2017Review
- Gap junctions and hemichannels: communicating cell death in neurodevelopment and disease.BMC cell biology · 2017Review
- Repression of Astrocytic Connexins in Cortical and Subcortical Brain Regions and Prefrontal Enrichment of H3K9me3 in Depression and Suicide.The international journal of neuropsychopharmacology · 2017Article
- Astrocytes As the Main Players in Primary Degenerative Disorders of the Human Central Nervous System.Frontiers in aging neuroscience · 2016Review
- Chemical-Free Technique to Study the Ultrastructure of Primary Cilium.Scientific reports · 2015Article
- Neuronal gap junction coupling as the primary determinant of the extent of glutamate-mediated excitotoxicity.Journal of neural transmission (Vienna, Austria : 1996) · 2014Review
- Amitriptyline up-regulates connexin43-gap junction in rat cultured cortical astrocytes via activation of the p38 and c-Fos/AP-1 signalling pathway.British journal of pharmacology · 2014Article
- Mix and match: investigating heteromeric and heterotypic gap junction channels in model systems and native tissues.FEBS letters · 2014Review
- Relevance of gap junctions and large pore channels in traumatic brain injury.Frontiers in physiology · 2014Review
- Modeling human neurodevelopmental disorders in the Xenopus tadpole: from mechanisms to therapeutic targets.Disease models & mechanisms · 2013Review
- Neuronal gap junctions: making and breaking connections during development and injury.Trends in neurosciences · 2013Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Despite the combination of light-microscopic immunocytochemistry, histochemical mRNA detection techniques and protein reporter systems, progress in identifying the protein composition of neuronal versus glial gap junctions, determination of the differential localization of their constituent connexin proteins in two apposing membranes and understanding human neurological diseases caused by connexin mutations has been problematic due to ambiguities introduced in the cellular and subcellular assignment of connexins. Misassignments occurred primarily because membranes and their constituent proteins are below the limit of resolution of light microscopic imaging techniques. Currently, only serial thin-section transmission electron microscopy and freeze-fracture replica immunogold labeling have sufficient resolution to assign connexin proteins to either or both sides of gap junction plaques. However, freeze-fracture replica immunogold labeling has been limited because conventional freeze fracturing allows retrieval of only one of the two membrane fracture faces within a gap junction, making it difficult to identify connexin coupling partners in hemiplaques removed by fracturing. We now summarize progress in ascertaining the connexin composition of two coupled hemiplaques using matched double-replicas that are labeled simultaneously for multiple connexins. This approach allows unambiguous identification of connexins and determination of the membrane "sidedness" and the identities of connexin coupling partners in homotypic and heterotypic gap junctions of vertebrate neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.