ArticleJournal of clinical microbiology2012
Detection of Kudoa septempunctata 18S ribosomal DNA in patient fecal samples from novel food-borne outbreaks caused by consumption of raw olive flounder (Paralichthys olivaceus).
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Molecular and genetic characterization of Kudoa septempunctata from foodborne outbreaks in the Republic of Korea, 2016-2024: a retrospective public-health surveillance investigation.Osong public health and research perspectives · 2026Article
- Epidemiology of Kudoa septempunctata food poisoning in Japan from 2013 to 2023.Scientific reports · 2026Article
- The association between the consumption of raw Kudoa septempunctata-infected farmed Paralichthys olivaceus and gastrointestinal symptoms.Epidemiology and health · 2026Article
- Article
- An update of the species of Myxosporea (Cnidaria, Myxozoa) described from Indian fish.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2023Review
- A review of food poisoning caused by local food in Japan.Journal of general and family medicine · 2021Review
- Description of myxosporeans (Cnidaria: Myxozoa) infecting the popular food fishFood and waterborne parasitology · 2020Article
- Immune-triggering effect of the foodborne parasite Kudoa septempunctata through the C-type lectin Mincle in HT29 cells.BMB reports · 2020Article
- Incidence of three Kudoa spp., K. neothunni, K. hexapunctata, and K. thunni (Myxosporea: Multivalvulida), in Thunnus tunas distributed in the western Pacific Ocean.Parasitology research · 2017Article
- Repatriation of an old fish host as an opportunity for myxozoan parasite diversity: The example of the allis shad, Alosa alosa (Clupeidae), in the Rhine.Parasites & vectors · 2016Article
- Article
- Survivability of Kudoa septempunctata in human intestinal conditions.Parasitology research · 2016Article
- Morphological and molecular genetic characterization of two Kudoa spp., K. musculoliquefaciens, and K. pleurogrammi n. sp. (Myxosporea: Multivalvulida), causing myoliquefaction of commercial marine fish.Parasitology research · 2016Article
- Three Unicapsula species (Myxosporea: Trilosporidae) of Asian marine fishes, including the description of Unicapsula setoensis n. sp. in the yellowfin goby (Acanthogobius flavimanus) from the Inland Sea of Japan.Parasitology research · 2014Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kudoa septempunctata is a newly identified myxosporean parasite of olive flounder (Paralichthys olivaceus) and a suspected causative agent of several food-borne gastroenteritis outbreaks in Japan. Here, we report the detection of K. septempunctata 18S ribosomal DNA in fecal samples of outbreak patients using an efficient method based on real-time PCR. We first performed a spiking experiment to assess whether our previously developed real-time PCR assay was applicable to detect K. septempunctata in feces. Simultaneously, we compared the relative extraction efficacy of K. septempunctata DNA using three commercial kits. Finally, our detection method was validated by testing 45 clinical samples obtained from 13 food-borne outbreaks associated with the consumption of raw flounder and 41 fecal samples from diarrhea patients epidemiologically unrelated to the ingestion of raw fish. We found that the FastDNA Spin Kit for Soil (MP Biomedicals) was the most efficient method for extracting K. septempunctata DNA from fecal samples. Using this kit, the detection limit of our real-time PCR assay was 1.6 × 10(1) spores per g of feces, and positive results were obtained for 21 fecal and 2 vomitus samples obtained from the food-borne outbreaks. To our knowledge, this is the first report to describe the detection of K. septempunctata DNA in patient fecal samples. We anticipate that our detection method will be useful for confirming food-borne diseases caused by K. septempunctata in laboratory investigations.
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