ArticleRNA (New York, N.Y.)2012
Sequential RNA degradation pathways provide a fail-safe mechanism to limit the accumulation of unspliced transcripts in Saccharomyces cerevisiae.
Article in RNA (New York, N.Y.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Transcription termination promotes splicing efficiency and fidelity in a compact genome.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Emerging and re-emerging themes in co-transcriptional pre-mRNA splicing.Molecular cell · 2024Review
- Truncating the spliceosomal 'rope protein' Prp45 results in Htz1 dependent phenotypes.RNA biology · 2024Article
- Splicing inactivation generates hybrid mRNA-snoRNA transcripts targeted by cytoplasmic RNA decay.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Nuclear mRNA Quality Control and Cytoplasmic NMD Are Linked by the Guard Proteins Gbp2 and Hrb1.International journal of molecular sciences · 2021Review
- Folding Stability of Pax9 Intronic G-Quadruplex Correlates with Relative Molar Size in Eutherians.Molecular biology and evolution · 2021Article
- RNA exosome mutations in pontocerebellar hypoplasia alter ribosome biogenesis and p53 levels.Life science alliance · 2020Article
- A negative feedback mechanism links UBC gene expression to ubiquitin levels by affecting RNA splicing rather than transcription.Scientific reports · 2019Article
- Variants in EXOSC9 Disrupt the RNA Exosome and Result in Cerebellar Atrophy with Spinal Motor Neuronopathy.American journal of human genetics · 2018Article
- The histone variant H2A.Z promotes efficient cotranscriptional splicing inGenes & development · 2017Article
- Mutations of EXOSC3/Rrp40p associated with neurological diseases impact ribosomal RNA processing functions of the exosome inRNA (New York, N.Y.) · 2017Article
- Transcriptome-wide identification of the RNA-binding landscape of the chromatin-associated protein PARP1 reveals functions in RNA biogenesis.Cell discovery · 2017Article
- Splicing-Mediated Autoregulation Modulates Rpl22p Expression in Saccharomyces cerevisiae.PLoS genetics · 2016Article
- The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases.RNA (New York, N.Y.) · 2016Article
- Yeast Coq9 controls deamination of coenzyme Q intermediates that derive from para-aminobenzoic acid.Biochimica et biophysica acta · 2015Article
- Widespread exon skipping triggers degradation by nuclear RNA surveillance in fission yeast.Genome research · 2015Article
- Article
- Stress-induced nuclear RNA degradation pathways regulate yeast bromodomain factor 2 to promote cell survival.PLoS genetics · 2014Article
- Regulation of natural mRNAs by the nonsense-mediated mRNA decay pathway.Eukaryotic cell · 2014Review
- Global analysis of the nuclear processing of transcripts with unspliced U12-type introns by the exosome.Nucleic acids research · 2014Article
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2 authors.
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Abstract
The nuclear exosome and the nonsense-mediated mRNA decay (NMD) pathways have been implicated in the degradation of distinct unspliced transcripts in Saccharomyces cerevisiae. In this study we show that these two systems can act sequentially on specific unspliced pre-mRNAs to limit their accumulation. Using steady-state and decay analyses, we show that while specific unspliced transcripts rely mostly on NMD or on the nuclear exosome for their degradation, some unspliced RNAs are stabilized only when both the nuclear exosome and NMD are inactivated. We found that the mechanism of degradation of these unspliced pre-mRNAs is not influenced by promoter identity. However, the specificity in the pre-mRNAs degradation pathways can be manipulated by changing the rate of export or retention of these mRNAs. For instance, reducing the nuclear export of pre-mRNAs mostly degraded by NMD results in a higher fraction of unspliced transcripts degraded by the nuclear exosome. Reciprocally, inactivating the Mlp retention factors results in a higher fraction of unspliced transcripts degraded by NMD for precursors normally targeted by the nuclear exosome. Overall, these results demonstrate that a functional redundancy exists between nuclear and cytoplasmic degradation pathways for unspliced pre-mRNAs, and suggest that the degradation routes of these species are mainly determined by the efficiency of their nuclear export rates. The presence of these two sequential degradation pathways for unspliced pre-mRNAs underscores the importance of limiting their accumulation and might serve as a fail-safe mechanism to prevent the expression of these nonfunctional RNAs.
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