ArticleProceedings of the National Academy of Sciences of the United States of America2012
Confinement induces actin flow in a meiotic cytoplasm.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Filament transport supports contractile steady states of actin networks.bioRxiv : the preprint server for biology · 2025Article
- Density-dependent flow generation in active cytoskeletal fluids.Scientific reports · 2024Article
- How cytoskeletal crosstalk makes cells move: Bridging cell-free and cell studies.Biophysics reviews · 2024Review
- Artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction-diffusion waves.Biophysics and physicobiology · 2024Review
- Recycling of the actin monomer pool limits the lifetime of network turnover.The EMBO journal · 2023Article
- Unveiling the physics underlying symmetry breaking of the actin cytoskeleton: An artificial cell-based approach.Biophysics and physicobiology · 2023Article
- Geometric trade-off between contractile force and viscous drag determines the actomyosin-based motility of a cell-sized droplet.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Review
- Tug-of-war between actomyosin-driven antagonistic forces determines the positioning symmetry in cell-sized confinement.Nature communications · 2020Article
- Article
- Cytoskeleton Force Exertion in Bulk Cytoplasm.Frontiers in cell and developmental biology · 2020Review
- Scaling behaviour in steady-state contracting actomyosin networks.Nature physics · 2019Article
- Spatial confinement of active microtubule networks induces large-scale rotational cytoplasmic flow.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Probing the biology of cell boundary conditions through confinement of Xenopus cell-free cytoplasmic extracts.Genesis (New York, N.Y. : 2000) · 2017Review
- Xenopus extract approaches to studying microtubule organization and signaling in cytokinesis.Methods in cell biology · 2017Article
- Triggering signaling pathways using F-actin self-organization.Scientific reports · 2016Article
- Use of Xenopus cell-free extracts to study size regulation of subcellular structures.The International journal of developmental biology · 2016Review
- Reconstitution of self-organizing protein gradients as spatial cues in cell-free systems.eLife · 2014Article
- Actin-myosin spatial patterns from a simplified isotropic viscoelastic model.Biophysical journal · 2014Article
- Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vivo, F-actin flows are observed at different cell life stages and participate in various developmental processes during asymmetric divisions in vertebrate oocytes, cell migration, or wound healing. Here, we show that confinement has a dramatic effect on F-actin spatiotemporal organization. We reconstitute in vitro the spontaneous generation of F-actin flow using Xenopus meiotic extracts artificially confined within a geometry mimicking the cell boundary. Perturbations of actin polymerization kinetics or F-actin nucleation sites strongly modify the network flow dynamics. A combination of quantitative image analysis and biochemical perturbations shows that both spatial localization of F-actin nucleators and actin turnover play a decisive role in generating flow. Interestingly, our in vitro assay recapitulates several symmetry-breaking processes observed in oocytes and early embryonic cells.
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