ArticleProceedings of the National Academy of Sciences of the United States of America2012
Transcriptional regulation of juvenile hormone-mediated induction of Krüppel homolog 1, a repressor of insect metamorphosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 150 papers, 1 of them a synthesis that pooled it.
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Who cites it
150 citing papers in PubMed, 1 synthesis or guideline pooled it, 334 citations in OpenAlex.
- Advances in the study of juvenile hormone signaling.Frontiers in physiology · 2026Pooled it
- Article
- Juvenile hormone receptor relies on conserved domain interfaces for dimerization-dependent activity.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Juvenile hormone-mediated chromatin remodelling by cumulative action of downstream transcription factors, Hairy and Krüppel homolog 1.Communications biology · 2026Article
- Genome-Wide Identification of Transcriptional Start Sites and Candidate Enhancers Regulating Worker Metamorphosis inInsects · 2026Article
- Two cytochrome P450 epoxidases mediate juvenile hormone biosynthesis in Drosophila melanogaster.Insect biochemistry and molecular biology · 2026Article
- Characterization of the Crustacean Methyl Farnesoate Transcriptional Signaling Genes.International journal of molecular sciences · 2026Article
- Evolution of environmental sex determination via juvenile hormone-induced gene co-option inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- MET goes to the membrane-A new place for the "old" juvenile hormone receptor.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Involvement of Taiman in juvenile hormone signaling controlling sexual maturation in a male moth.Current research in insect science · 2026Article
- Two cytochrome P450 epoxidases mediate juvenile hormone biosynthesis inbioRxiv : the preprint server for biology · 2025Article
- Adult nutrient shortage impairs female reproduction via the attenuated juvenile hormone signaling during vitellogenesis in Helicoverpa armigera (Lepidoptera: Noctuidae).Journal of insect science (Online) · 2025Article
- SUMOylation modulates the dual functions of Krüppel homolog 1 in transcriptional regulation of Broad-Complex expression.Journal of advanced research · 2025Article
- 20E-inducedProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Article
- Interactions of juvenile hormone, 20-hydroxyecdysone, developmental genes, and miRNAs during pupal development in Apis mellifera.Scientific reports · 2025Article
- The PBAP chromatin remodeling complex mediates summer diapause via H3K4me3-driven juvenile hormone regulation inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- The seminal vesicle is a juvenile hormone-responsive tissue in adult maleOpen biology · 2024Article
90 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Krüppel homolog 1 gene (Kr-h1) has been proposed to play a key role in the repression of insect metamorphosis. Kr-h1 is assumed to be induced by juvenile hormone (JH) via a JH receptor, methoprene-tolerant (Met), but the mechanism of induction is unclear. To elucidate the molecular mechanism of Kr-h1 induction, we first cloned cDNAs encoding Kr-h1 (BmKr-h1) and Met (BmMet1 and BmMet2) homologs from Bombyx mori. In a B. mori cell line, BmKr-h1 was rapidly induced by subnanomolar levels of natural JHs. Reporter assays identified a JH response element (kJHRE), comprising 141 nucleotides, located ∼2 kb upstream from the BmKr-h1 transcription start site. The core region of kJHRE (GGCCTCCACGTG) contains a canonical E-box sequence to which Met, a basic helix-loop-helix Per-ARNT-Sim (bHLH-PAS) transcription factor, is likely to bind. In mammalian HEK293 cells, which lack an intrinsic JH receptor, ectopic expression of BmMet2 fused with Gal4DBD induced JH-dependent activity of an upstream activation sequence reporter. Meanwhile, the kJHRE reporter was activated JH-dependently in HEK293 cells only when cotransfected with BmMet2 and BmSRC, another bHLH-PAS family member, suggesting that BmMet2 and BmSRC jointly interact with kJHRE. We also found that the interaction between BmMet2 and BmSRC is dependent on JH. Therefore, we propose the following hypothesis for the mechanism of JH-mediated induction of BmKr-h1: BmMet2 accepts JH as a ligand, JH-liganded BmMet2 interacts with BmSRC, and the JH/BmMet2/BmSRC complex activates BmKr-h1 by interacting with kJHRE.
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