ArticleInfection and immunity2012
Identification of genes important for growth of asymptomatic bacteriuria Escherichia coli in urine.
Article in Infection and immunity, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 0 citations in OpenAlex.
- The Homoserine DehydrogenaseMicroorganisms · 2026Article
- Effect of Bladder Catheterization On Bacterial Interference With Asymptomatic Escherichia coli Strain 83972 in an Experimental Porcine Model of Urinary Tract Infection.The Journal of infectious diseases · 2025Article
- Less is more: the lack of autoinducer-2-dependent quorum sensing promotes competitive fitness ofFrontiers in cellular and infection microbiology · 2025Article
- Competitive fitness of asymptomatic bacteriuriaInfection and immunity · 2024Article
- The Role of Metabolomics and Microbiology in Urinary Tract Infection.International journal of molecular sciences · 2024Review
- Colistin-resistance genes in Escherichia coli isolated from patients with urinary tract infections.PloS one · 2024Article
- Metabolomics survey of uropathogenic bacteria in human urine.Frontiers in microbiology · 2024Article
- Bacterial Growth of Uropathogenic Escherichia coli in Pooled Urine Is Much Higher than Predicted from the Average Growth in Individual Urine Samples.Microbiology spectrum · 2022Article
- Dihydroxy-Acid Dehydratases From Pathogenic Bacteria: Emerging Drug Targets to Combat Antibiotic Resistance.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Adaptive strategies of uropathogenic Escherichia coli CFT073: from growth in lab media to virulence during host cell adhesion.International microbiology : the official journal of the Spanish Society for Microbiology · 2022Article
- Proteomes of UropathogenicProteomes · 2022Article
- "Omics" Technologies - What Have They Told Us About UropathogenicFrontiers in cellular and infection microbiology · 2022Review
- Genome-wide analysis of fitness-factors in uropathogenicMicrobial genomics · 2021Article
- Article
- Rapid Growth and Metabolism of Uropathogenic Escherichia coli in Relation to Urine Composition.Clinical microbiology reviews · 2019Review
- Amino Acid Biosynthetic Pathways Are Required forAntimicrobial agents and chemotherapy · 2019Article
- Frequency of bacteriuria in dogs with chronic kidney disease: A retrospective study of 201 cases.Journal of veterinary internal medicine · 2019Article
- Targeting Metalloenzymes for Therapeutic Intervention.Chemical reviews · 2019Review
- The impact of inactivation of the purine biosynthesis genes, purN and purT, on growth and virulence in uropathogenic E. coli.Molecular biology reports · 2018Article
- Evaluation of E. coli inhibition by plain and polymer-coated silver nanoparticles.Revista do Instituto de Medicina Tropical de Sao Paulo · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli is the most important etiological agent of urinary tract infections (UTIs). Unlike uropathogenic E. coli, which causes symptomatic infections, asymptomatic bacteriuria (ABU) E. coli strains typically lack essential virulence factors and colonize the bladder in the absence of symptoms. While ABU E. coli can persist in the bladder for long periods of time, little is known about the genetic determinants required for its growth and fitness in urine. To identify such genes, we have employed a transposon mutagenesis approach using the prototypic ABU E. coli strain 83972 and the clinical ABU E. coli strain VR89. Six genes involved in the biosynthesis of various amino acids and nucleobases were identified (carB, argE, argC, purA, metE, and ilvC), and site-specific mutants were subsequently constructed in E. coli 83972 and E. coli VR89 for each of these genes. In all cases, these mutants exhibited reduced growth rates and final cell densities in human urine. The growth defects could be complemented in trans as well as by supplementation with the appropriate amino acid or nucleobase. When assessed in vivo in a mouse model, E. coli 83972carAB and 83972argC showed a significantly reduced competitive advantage in the bladder and/or kidney during coinoculation experiments with the parent strain, whereas 83972metE and 83972ilvC did not. Taken together, our data have identified several biosynthesis pathways as new important fitness factors associated with the growth of ABU E. coli in human urine.
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