Evidence map›Paper›PMID 22752953›Full record

ArticleCurrent protocols in cytometry2012

Ex vivo imaging of excised tissue using vital dyes and confocal microscopy.

Simon Johnson, Peter Rabinovitch

Abstract read
In one paragraph

Article in Current protocols in cytometry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed.

  1. Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy.Journal of visualized experiments : JoVE · 2025
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  9. Understanding the sources of errors inBiomedical optics express · 2021
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  11. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Simon JohnsonUniversity of Washington, Seattle, Washington, USA.
Peter Rabinovitch

Funding

SPECIFICITY OF OXYGEN DNA DAMAGE AND MUTAGENESISP01AG001751 · NIA · UNIVERSITY OF WASHINGTON · PI RABINOVITCH, PETER S · 1985 to 2021
$29.6M
University of Washington Nathan Shock Center of Excellence in the Basic Biology of AgingP30AG013280 · NIA · UNIVERSITY OF WASHINGTON · PI Su-In Lee · 1995 to 2026
$27.1M
GENETIC APPROACHES TO AGING RESEARCHT32AG000057 · NIA · UNIVERSITY OF WASHINGTON · PI RABINOVITCH, PETER S · 1985 to 2019
$14.2M
Cardiomyocyte mitochondria and mtROS in cardiac aging, hypertrophy and failureR01HL101186 · NHLBI · UNIVERSITY OF WASHINGTON · PI RABINOVITCH, PETER S · 2010 to 2013
$2.5M
NHLBI NIH HHS R01 HL101186NIA NIH HHS P01 AG001751NIA NIH HHS P30 AG013280NIA NIH HHS T32 AG000057
6 · The paper itself

Abstract

Vital dyes routinely used for staining cultured cells can also be used to stain and image live tissue slices ex vivo. Staining tissue with vital dyes allows researchers to collect structural and functional data simultaneously and can be used for qualitative or quantitative fluorescent image collection. The protocols presented here are useful for structural and functional analysis of viable properties of cells in intact tissue slices, allowing for the collection of data in a structurally relevant environment. With these protocols, vital dyes can be applied as a research tool to disease processes and properties of tissue not amenable to cell culture-based studies.

Indexed as

Organ SpecificityAnimalsCell NucleusCell SurvivalColoring AgentsData CollectionEthidiumFluoresceinsGreen Fluorescent ProteinsImaging, Three-DimensionalMiceMicroscopy, ConfocalMitochondriaOrganic ChemicalsOrganometallic CompoundsStaining and Labelingcalcein AMColoring AgentsEthidiumethidium homodimerFluoresceinsGreen Fluorescent ProteinsOrganic ChemicalsOrganometallic CompoundsSYTOX Greentetramethyl rhodamine ethyl ester

Identifiers

PMID22752953
PMCPMC3401092

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.