ArticleCurrent protocols in neuroscience2012
Direct bisulfite sequencing for examination of DNA methylation with gene and nucleotide resolution from brain tissues.
Article in Current protocols in neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 2 of them syntheses that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Advancing Forensic Human Chronological Age Estimation: Biochemical, Genetic, and Epigenetic Approaches from the Last 15 Years: A Systematic Review.International journal of molecular sciences · 2025Pooled it
- Guidelines for pre-analytical conditions for assessing the methylation of circulating cell-free DNA.Clinical epigenetics · 2021Pooled it
- Promoter hypomethylation drives ABCB1-mediated carfilzomib resistance in multiple myeloma.Clinical epigenetics · 2026Article
- Review
- Development and Clinical Validation of Molecular Subgrouping in Medulloblastoma by Targeted Methylation Sequencing.Asian Pacific journal of cancer prevention : APJCP · 2025Article
- Promoter Methylation of HIV Coreceptor-Related Genes CCR5 and CXCR4: Original Research.Viruses · 2025Article
- A procedure for DNA methylation assessment in osteoporosis-related gene promoters of umbilical cord blood: A study on the Prospective Epidemiological Research Studies in Iran (PERSIAN) birth cohort.BioImpacts : BI · 2025Article
- Article
- Alterations in DNA 5-hydroxymethylation patterns in the hippocampus of an experimental model of chronic epilepsy.Neurobiology of disease · 2024Article
- Correlation of antigen expression with epigenetic modifications after rAAV delivery of a human factor IX variant in mice and rhesus macaques.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
- ABSP: an automated R tool to efficiently analyze region-specific CpG methylation from bisulfite sequencing PCR.Bioinformatics (Oxford, England) · 2023Article
- DNA methylation and expression of the egfr gene are associated with worker size in monomorphic ants.Scientific reports · 2022Article
- Methylation in the Promoter Region of the Dopamine TransporterInternational journal of environmental research and public health · 2022Article
- The Epigenetic Regulation in Plant Specialized Metabolism: DNA Methylation Limits PaclitaxelFrontiers in plant science · 2022Article
- Exposure of pigs to glyphosate affects gene-specific DNA methylation and gene expression.Toxicology reports · 2022Article
- Article
- Epigenetic effects of Bisphenol A on granulosa cells of mouse follicles during in vitro culture: An experimental study.International journal of reproductive biomedicine · 2021Article
- DNA methylation and behavioral changes induced by neonatal spinal transection.Infant behavior & development · 2019Article
- Predictive MGMT status in a homogeneous cohort of IDH wildtype glioblastoma patients.Acta neuropathologica communications · 2019Article
- Methylation analysis of HOXA10 regulatory elements in patients with endometriosis.BMC research notes · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
DNA methylation is an epigenetic modification that is essential for the development and mature function of the central nervous system. Due to the relevance of this modification to the transcriptional control of gene expression, it is often necessary to examine changes in DNA methylation patterns with both gene and single-nucleotide resolution. Here, we describe an in-depth basic protocol for direct bisulfite sequencing of DNA isolated from brain tissue, which will permit direct assessment of methylation status at individual genes as well as individual cytosine molecules/nucleotides within a genomic region. This method yields analysis of DNA methylation patterns that is robust, accurate, and reproducible, thereby allowing insights into the role of alterations in DNA methylation in brain tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.