ArticleThe Journal of membrane biology2012
Neurons and β-cells of the pancreas express connexin36, forming gap junction channels that exhibit strong cationic selectivity.
Article in The Journal of membrane biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Defining the electrical synapse.Nature reviews. Neuroscience · 2026Review
- Connexin Gap Junction Channels and Hemichannels: Insights from High-Resolution Structures.Biology · 2024Review
- Cryo-EM structures of human Cx36/GJD2 neuronal gap junction channel.Nature communications · 2023Article
- Review
- Small subpopulations of β-cells do not drive islet oscillatory [Ca2+] dynamics via gap junction communication.PLoS computational biology · 2021Article
- Gap junctions coordinate the propagation of glycogenolysis induced by norepinephrine in the pineal gland.Journal of neurochemistry · 2019Article
- Connexins-Based Hemichannels/Channels and Their Relationship with Inflammation, Seizures and Epilepsy.International journal of molecular sciences · 2019Review
- Midbody: From the Regulator of Cytokinesis to Postmitotic Signaling Organelle.Medicina (Kaunas, Lithuania) · 2018Review
- Electrical synapses in mammalian CNS: Past eras, present focus and future directions.Biochimica et biophysica acta. Biomembranes · 2018Review
- Connexin36 localization to pinealocytes in the pineal gland of mouse and rat.The European journal of neuroscience · 2017Article
- A Variant of GJD2, Encoding for Connexin 36, Alters the Function of Insulin Producing β-Cells.PloS one · 2016Article
- Fluorescence recovery after photobleaching reveals regulation and distribution of connexin36 gap junction coupling within mouse islets of Langerhans.The Journal of physiology · 2014Article
- Two functionally distinct networks of gap junction-coupled inhibitory neurons in the thalamic reticular nucleus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Article
- Connexins and steroidogenesis in mouse Leydig cells.Canadian journal of physiology and pharmacology · 2013Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
We examined the permeability of connexin36 (Cx36) homotypic gap junction (GJ) channels, expressed in neurons and β-cells of the pancreas, to dyes differing in molecular mass and net charge. Experiments were performed in HeLa cells stably expressing Cx36 tagged with EGFP by combining a dual whole-cell voltage clamp and fluorescence imaging. To assess the permeability of the single GJ channel (P(γ)), we used a dual-mode excitation of fluorescent dyes that allowed us to measure cell-to-cell dye transfer at levels not resolvable using whole-field excitation solely. We demonstrate that P(γ) of Cx36 for cationic dyes (EAM-1⁺ and EAM-2⁺) is ~10-fold higher than that for an anionic dye of the same net charge and similar molecular mass, Alexa fluor-350 (AFl-350⁻). In addition, P(γ) for Lucifer yellow (LY²⁻) is approximately fourfold smaller than that for AFl-350⁻, which suggests that the higher negativity of LY²⁻ significantly reduces permeability. The P(γ) of Cx36 for AFl-350 is approximately 358, 138, 23 and four times smaller than the P(γ)s of Cx43, Cx40, Cx45, and Cx57, respectively. In contrast, it is 6.5-fold higher than the P(γ) of mCx30.2, which exhibits a smaller single-channel conductance. Thus, Cx36 GJs are highly cation-selective and should exhibit relatively low permeability to numerous vital negatively charged metabolites and high permeability to K⁺, a major charge carrier in cell-cell communication.
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