ArticleJournal of plant research2013
The carbohydrate-binding module of Fragaria × ananassa expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro".
Article in Journal of plant research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Hormone-regulated expansins: Expression, localization, and cell wall biomechanics in Arabidopsis root growth.Plant physiology · 2023Article
- Expansin-mediated developmental and adaptive responses: A matter of cell wall biomechanics?Quantitative plant biology · 2022Review
- Virtual issue: cell wall functions in plant growth and environmental responses.Journal of plant research · 2021Article
- Analysis of the Binding of Expansin Exl1, fromACS omega · 2018Article
- A Profusion of Molecular Scissors for Pectins: Classification, Expression, and Functions of Plant Polygalacturonases.Frontiers in plant science · 2018Review
- Growth modulation effects of CBM2a under the control of AtEXP4 and CaMV35S promoters in Arabidopsis thaliana, Nicotiana tabacum and Eucalyptus camaldulensis.Transgenic research · 2017Article
- Transcriptome Analysis of Maize Immature Embryos Reveals the Roles of Cysteine in ImprovingFrontiers in plant science · 2017Article
- Plant expansins: diversity and interactions with plant cell walls.Current opinion in plant biology · 2015Review
- In-silico analysis of the structure and binding site features of an α-expansin protein from mountain papaya fruit (VpEXPA2), through molecular modeling, docking, and dynamics simulation studies.Journal of molecular modeling · 2015Article
- Overexpression of the carbohydrate binding module of strawberry expansin2 in Arabidopsis thaliana modifies plant growth and cell wall metabolism.Plant molecular biology · 2015Article
- Bacterial expansins and related proteins from the world of microbes.Applied microbiology and biotechnology · 2015Review
- Over-expression of AtEXLA2 alters etiolated arabidopsis hypocotyl growth.Annals of botany · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
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Abstract
A putative carbohydrate binding module (CBM) from strawberry (Fragaria × ananassa Duch.) expansin 2 (CBM-FaExp2) was cloned and the encoding protein was over-expressed in Escherichia coli and purified in order to evaluate its capacity to bind different cell wall polysaccharides "in vitro". The protein CBM-FaExp2 bound to microcrystalline cellulose, xylan and pectin with different affinities (K(ad) = 33.6 ± 0.44 mL g(-1), K(ad) = 11.37 ± 0.87 mL g(-1), K(ad) = 10.4 ± 0.19 mL g(-1), respectively). According to "in vitro" enzyme assays, this CBM is able to decrease the activity of cell wall degrading enzymes such as polygalacturonase, endo-glucanase, pectinase and xylanase, probably because the binding of CBM-FaExp2 to the different substrates interferes with enzyme activity. The results suggest that expansins would bind not only cellulose but also a wide range of cell wall polymers.
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