Identification and characterization of myophilin-like protein: a life stage and tissue-specific antigen of Clonorchis sinensis.

Clonorchiasis, caused by Clonorchis sinensis infection, has been an important public health problem in China. More messages about biology and pathogenicity of C. sinensis will be better for development of new strategies for clonorchiasis control. In the current study, using data from the published genomic and cDNA sequences for C. sinensis, we identify a gene encoding a myophilin-like protein (Cs myophilin-like). The cDNA sequence is 573 bp in length which encoded a protein of 190 amino acids. DNA sequence of Cs myophilin-like consists of four extrons and three introns. By mapping the draft genome of C. sinensis, single copy of myophilin-like gene is annotated. Bioinformatic analysis shows Cs myophilin-like comprises a calponin homology domain and three actin binding surfaces. It expresses at the life stage of adult worm and metacercaria but not in eggs. It intensively distributes in muscular locations such as oral sucker and pharynx of adult worm and sucker of metacercaria. The transcript level of Cs myophilin-like in oral sucker and pharynx is significantly higher than that in the rest of adult worm. Moreover, recombinant Cs myophilin-like is obtained and of antigenicity and immunoreactivity. It can be probed by sera from patients infected with trematode or cestode. Our data suggests that Cs myophilin-like may be involved in the host tissue invasion besides regulating the contraction of smooth muscle and movement of worm body. Taken together, the stable and soluble expression in Escherichia coli and immunogenicity of recombinant Cs myophilin-like proteins suggest that Cs myophilin-like may be a potential candidate for vaccine and drug target for clonorchiasis.