ArticleIn vitro cellular & developmental biology. Animal2012
A cell line derived from the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae).
Article in In vitro cellular & developmental biology. Animal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 51 citations in OpenAlex.
- N(alpha)-acetyltransferase 40-mediated histone acetylation plays an important role in ecdysone regulation of metamorphosis in the red flour beetle, Tribolium castaneum.Communications biology · 2024Article
- Characterization of Iflavirus in the Red Flour Beetle,Insects · 2023Article
- Mitochondria dysfunction impairs Tribolium castaneum wing development during metamorphosis.Communications biology · 2022Article
- Establishing and characterising a new cell line fromHeliyon · 2022Article
- Isolation, establishment and characterization of new insect cell line derived from midgut of rice weevil, Sitophilus oryzae.In vitro cellular & developmental biology. Animal · 2020Article
- Histone deacetylase 3 is required for development and metamorphosis in the red flour beetle, Tribolium castaneum.BMC genomics · 2020Article
- Cell lines derived from the western corn rootworm larvae, Diabrotica virgifera virgifera (Chrysomelidae: Coleoptera).In vitro cellular & developmental biology. Animal · 2020Article
- Cell Lines for Honey Bee Virus Research.Viruses · 2020Review
- Histone Deacetylase 11 Knockdown Blocks Larval Development and Metamorphosis in the Red Flour Beetle,Frontiers in genetics · 2020Article
- Generation of Virus- and dsRNA-Derived siRNAs with Species-Dependent Length in Insects.Viruses · 2019Article
- Generating and working with Drosophila cell cultures: Current challenges and opportunities.Wiley interdisciplinary reviews. Developmental biology · 2019Review
- Epigenetic modifications acetylation and deacetylation play important roles in juvenile hormone action.BMC genomics · 2018Article
- Characterization of cell lines derived from the southern armyworm, Spodoptera eridania.In vitro cellular & developmental biology. Animal · 2018Article
- CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum.Scientific reports · 2018Article
- Expanding the genetic toolkit of Tribolium castaneum.PloS one · 2018Article
- Toxicity, membrane binding and uptake of the Sclerotinia sclerotiorum agglutinin (SSA) in different insect cell lines.In vitro cellular & developmental biology. Animal · 2017Article
- Toxicity of Naphthalene and Benzene on Tribollium castaneum Herbst.International journal of environmental research and public health · 2017Article
- Prostaglandin actions in established insect cell lines.In vitro cellular & developmental biology. Animal · 2017Article
- Cell lines derived from the squash bug, Anasa tristis (Coreidae: Hemiptera).In vitro cellular & developmental biology. Animal · 2017Article
- Atypical centrioles are present inOpen biology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The red flour beetle, Tribolium castaneum, is a model organism for agricultural and medical research and its complete genome is sequenced. We established a continuously replicating T. castaneum cell line to complement existing physiological, genetic, and genomic research tools. We set up trial cell cultures from egg, pupa, and adult stages as tissue sources and incubated them in six separate cell culture media to determine the optimal combination of tissue source and medium for cell replication. Our most promising culture was generated by co-culturing adult (∼75 %) and pupal tissues in EX-CELL 420 medium containing 9 % FBS. Our new cell culture is designated BCIRL-TcA-CLG1 (TcA) and it has been subcultured more than 90 times. Amplification of genomic DNA with species-specific primers yielded DNA fragments of the expected sizes and with sequences identical to those from the published Tribolium genome. Additionally, we characterized this line using DNA fingerprinting (DAF-PCR) and compared it with three other coleopteran cell lines and its conspecific pupae to confirm identity. Its doubling time is 155.2 hr. Early passages consisted of attached cells and vesicles in suspension, whereas later passages consisted primarily of attached, spherical cells. Similar to other established cell lines, the ploidy of TcA cells was variable, ranging from 20 chromosomes/cell (diploid) to above 30 chromosomes/cell. TcA cells withstood incubation at 40°C for 1 h with no decrease in viability. We recorded increased levels of one heat shock protein (43 kDa) and of the hsp68a transcript following exposure to 40°C. Taken together, this represents the first report of a continuously replicating T. castaneum cell line. We expect the BCIRL-TcA-CLG1 line will become a useful tool in Tribolium research.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.