ArticleApplied and environmental microbiology2012
Internalization of sapovirus, a surrogate for norovirus, in romaine lettuce and the effect of lettuce latex on virus infectivity.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 37 citations in OpenAlex.
- SARS-CoV-2 remains infectious for at least a month on artificially-contaminated frozen berries.Food microbiology · 2022Article
- Tissue Distribution and Visualization of Internalized Human Norovirus in Leafy Greens.Applied and environmental microbiology · 2018Article
- Internalization and dissemination of human norovirus and Tulane virus in fresh produce is plant dependent.Food microbiology · 2018Article
- Effect of Leaf Surface Chemical Properties on Efficacy of Sanitizer for Rotavirus Inactivation.Applied and environmental microbiology · 2016Article
- Survival and Transfer of Murine Norovirus within a Hydroponic System during Kale and Mustard Microgreen Harvesting.Applied and environmental microbiology · 2016Article
- Abiotic Stress and Phyllosphere Bacteria Influence the Survival of Human Norovirus and Its Surrogates on Preharvest Leafy Greens.Applied and environmental microbiology · 2016Article
- Feline Calicivirus, Murine Norovirus, Porcine Sapovirus, and Tulane Virus Survival on Postharvest Lettuce.Applied and environmental microbiology · 2015Article
- Possible Internalization of an Enterovirus in Hydroponically Grown Lettuce.International journal of environmental research and public health · 2015Article
- Effects of Abiotic and Biotic Stresses on the Internalization and Dissemination of Human Norovirus Surrogates in Growing Romaine Lettuce.Applied and environmental microbiology · 2015Article
- Efficacy and mechanisms of murine norovirus inhibition by pulsed-light technology.Applied and environmental microbiology · 2015Article
- Evidence of the internalization of animal caliciviruses via the roots of growing strawberry plants and dissemination to the fruit.Applied and environmental microbiology · 2015Article
- Influence of Epicuticular Physicochemical Properties on Porcine Rotavirus Adsorption to 24 Leafy Green Vegetables and Tomatoes.PloS one · 2015Article
- Comprehensive comparison of cultivable norovirus surrogates in response to different inactivation and disinfection treatments.Applied and environmental microbiology · 2014Article
- Mechanisms of antiviral action of plant antimicrobials against murine norovirus.Applied and environmental microbiology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Noroviruses are the leading cause of food-borne outbreaks, including those that involve lettuce. The culturable porcine sapovirus (SaV) was used as a norovirus surrogate to study the persistence and the potential transfer of the virus from roots to leaves and from outer to inner leaves of lettuce plants. Treatment of lettuce with SaV was done through the roots of young plants, the soil, or the outer leaves of mature plants. Sampling of roots, xylem sap, and inner and outer leaves followed by RNA extraction and SaV-specific real-time reverse transcription (RT)-PCR was performed at 2 h and on postinoculation days (PID) 2, 5, 7, 14, and/or 28. When SaV was inoculated through the roots, viral RNA persisted on the roots and in the leaves until PID 28. When the virus was inoculated through the soil, viral RNA was detected on the roots and in the xylem sap until PID 14; viral RNA was detected in the leaves only until PID 2. No infectious virus was detected inside the leaves for either treatment. When SaV was inoculated through the outer leaves, viral RNA persisted on the leaves until PID 14; however, the virus did not transfer to inner leaves. Infectious viral particles on leaves were detected only at 2 h postinoculation. The milky sap (latex) of leaves, but not the roots' xylem sap, significantly decreased virus infectivity when tested in vitro. Collectively, our results showed the transfer of SaV from roots to leaves through the xylem system and the capacity of the sap of lettuce leaves to decrease virus infectivity in leaves.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.