ArticleApplied and environmental microbiology2012
Protection of Bacillus pumilus spores by catalases.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
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- New Thoughts on an Old Topic: Secrets of Bacterial Spore Resistance Slowly Being Revealed.Microbiology and molecular biology reviews : MMBR · 2023Review
- In silico analysis of sporulene biosynthesis pathway genes in the members of the class Bacilli.Archives of microbiology · 2023Article
- Monofunctional Heme-Catalases.Antioxidants (Basel, Switzerland) · 2022Review
- Optimization of sporulation and purification methods for sporicidal efficacy assessment on Bacillus spores.Journal of industrial microbiology & biotechnology · 2022Article
- Laccase Properties, Physiological Functions, and Evolution.International journal of molecular sciences · 2020Review
- Bacillus megaterium SF185 spores exert protective effects against oxidative stress in vivo and in vitro.Scientific reports · 2019Article
- Article
- Sterilization of hydrogen peroxide resistant bacterial spores with stabilized chlorine dioxide.AMB Express · 2015Article
- An ICEBs1-like element may be associated with the extreme radiation and desiccation resistance of Bacillus pumilus SAFR-032 spores.Extremophiles : life under extreme conditions · 2013Article
- Utilization of low-pressure plasma to inactivate bacterial spores on stainless steel screws.Astrobiology · 2013Article
- Candidate genes that may be responsible for the unusual resistances exhibited by Bacillus pumilus SAFR-032 spores.PloS one · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus pumilus SAFR-032, isolated at spacecraft assembly facilities of the National Aeronautics and Space Administration Jet Propulsion Laboratory, is difficult to kill by the sterilization method of choice, which uses liquid or vapor hydrogen peroxide. We identified two manganese catalases, YjqC and BPUM_1305, in spore protein extracts of several B. pumilus strains by using PAGE and mass spectrometric analyses. While the BPUM_1305 catalase was present in six of the B. pumilus strains tested, YjqC was not detected in ATCC 7061 and BG-B79. Furthermore, both catalases were localized in the spore coat layer along with laccase and superoxide dismutase. Although the initial catalase activity in ATCC 7061 spores was higher, it was less stable over time than the SAFR-032 enzyme. We propose that synergistic activity of YjqC and BPUM_1305, along with other coat oxidoreductases, contributes to the enhanced resistance of B. pumilus spores to hydrogen peroxide. We observed that the product of the catalase reaction, gaseous oxygen, forms expanding vesicles on the spore surface, affecting the mechanical integrity of the coat layer, resulting in aggregation of the spores. The accumulation of oxygen gas and aggregations may play a crucial role in limiting further exposure of Bacilli spore surfaces to hydrogen peroxide or other toxic chemicals when water is present.
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