ArticleGenes & development2012
Synthetic memory circuits for tracking human cell fate.
Article in Genes & development, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 76 citations in OpenAlex.
- An epigenetic memory at the CYP1A gene in cancer-resistant, pollution-adapted killifish.Scientific reports · 2025Article
- Innovative applications and research advances of bacterial biosensors in medicine.Frontiers in microbiology · 2025Review
- An epigenetic memory at thebioRxiv : the preprint server for biology · 2024Article
- Design and mathematical analysis of activating transcriptional amplifiers that enable modular temporal control in synthetic juxtacrine circuits.Synthetic and systems biotechnology · 2023Article
- Origin, heterogeneity, and interconversion of noncanonical bistable switches from the positive feedback loops under dual signaling.iScience · 2023Article
- Scalable biological signal recording in mammalian cells using Cas12a base editors.Nature chemical biology · 2022Article
- The roles of inducible chromatin and transcriptional memory in cellular defense system responses to redox-active pollutants.Free radical biology & medicine · 2021Review
- Protein assembly systems in natural and synthetic biology.BMC biology · 2020Review
- Synthetic biology for improving cell fate decisions and tissue engineering outcomes.Emerging topics in life sciences · 2019Article
- Reprogramming the brain with synthetic neurobiology.Current opinion in biotechnology · 2019Review
- Towards control of cellular decision-making networks in the epithelial-to-mesenchymal transition.Physical biology · 2019Review
- Drug treatment efficiency depends on the initial state of activation in nonlinear pathways.Scientific reports · 2018Article
- Designing cell function: assembly of synthetic gene circuits for cell biology applications.Nature reviews. Molecular cell biology · 2018Review
- Bottom-up approaches in synthetic biology and biomaterials for tissue engineering applications.Journal of industrial microbiology & biotechnology · 2018Article
- Mammalian synthetic biology for studying the cell.The Journal of cell biology · 2017Review
- Survival of Phenotypic Information during Cellular Growth Transitions.ACS synthetic biology · 2016Article
- Molecular recordings by directed CRISPR spacer acquisition.Science (New York, N.Y.) · 2016Article
- Engineering Gene Circuits for Mammalian Cell-Based Applications.Cold Spring Harbor perspectives in biology · 2016Review
- Quantitative Analyses of Core Promoters Enable Precise Engineering of Regulated Gene Expression in Mammalian Cells.ACS synthetic biology · 2016Article
- Stress-response balance drives the evolution of a network module and its host genome.Molecular systems biology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A variety of biological phenomena, from disease progression to stem cell differentiation, are typified by a prolonged cellular response to a transient environmental cue. While biologically relevant, heterogeneity in these long-term responses is difficult to assess at the population level, necessitating the development of biological tools to track cell fate within subpopulations. Here we present a novel synthetic biology approach for identifying and tracking mammalian cell subpopulations. We constructed three genomically integrated circuits that use bistable autoregulatory transcriptional feedback to retain memory of exposure to brief stimuli. These "memory devices" are used to isolate and track the progeny of cells that responded differentially to doxycycline, hypoxia, or DNA-damaging agents. Following hypoxic or ultraviolet radiation exposure, strongly responding cells activate the memory device and exhibit changes in gene expression, growth rates, and viability for multiple generations after the initial stimulus. Taken together, these results indicate that a heritable memory of hypoxia and DNA damage exists in subpopulations that differ in long-term cell behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.