ArticleCell cycle (Georgetown, Tex.)2012
Aurora B regulates spindle bipolarity in meiosis in vertebrate oocytes.
Article in Cell cycle (Georgetown, Tex.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Molecular Mechanisms of Negative Effect of Systemic Lupus Erythematosus on Oogenesis and Meiotic Processes in Oocytes.International journal of molecular sciences · 2026Review
- Mutation of the SUMOylation site of Aurora-B disrupts spindle formation and chromosome alignment in oocytes.Cell death discovery · 2024Article
- EGFR-mediated hyperacetylation of tubulin induced docetaxel resistance by downregulation of HDAC6 and upregulation of MCAK and PLK1 in prostate cancer cells.The Kaohsiung journal of medical sciences · 2024Article
- Inositol 1, 4, 5-trisphosphate receptor is required for spindle assembly inMolecular biology of the cell · 2022Article
- Compromised MPS1 Activity Induces Multipolar Spindle Formation in Oocytes From Aged Mares: Establishing the Horse as a Natural Animal Model to Study Age-Induced Oocyte Meiotic Spindle Instability.Frontiers in cell and developmental biology · 2021Article
- Toxoplasma gondii chromosomal passenger complex is essential for the organization of a functional mitotic spindle: a prerequisite for productive endodyogeny.Cellular and molecular life sciences : CMLS · 2018Article
- Living Xenopus oocytes, eggs, and embryos as models for cell division.Methods in cell biology · 2018Article
- Spindle function in Xenopus oocytes involves possible nanodomain calcium signaling.Molecular biology of the cell · 2016Article
- KLP-7 acts through the Ndc80 complex to limit pole number in C. elegans oocyte meiotic spindle assembly.The Journal of cell biology · 2015Article
- Functional analysis of phosphorylation of the mitotic centromere-associated kinesin by Aurora B kinase in human tumor cells.Cell cycle (Georgetown, Tex.) · 2015Article
- Meiosis I in Xenopus oocytes is not error-prone despite lacking spindle assembly checkpoint.Cell cycle (Georgetown, Tex.) · 2014Article
- Xenopus oocyte meiosis lacks spindle assembly checkpoint control.The Journal of cell biology · 2013Article
- Calmodulin protects Aurora B on the midbody to regulate the fidelity of cytokinesis.Cell cycle (Georgetown, Tex.) · 2013Article
- Expression and cellular distribution of INHA and INHB before and after in vitro cultivation of porcine oocytes isolated from follicles of different size.Journal of biomedicine & biotechnology · 2012Article
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
Aurora B (Aur-B) plays multiple roles in mitosis, of which the best known are to ensure bi-orientation of sister chromatids by destabilizing incorrectly attached kinetochore microtubules and to participate in cytokinesis. Studies in Xenopus egg extracts, however, have indicated that Aur-B and the chromosome passenger complex play an important role in stabilizing chromosome-associated spindle microtubules. Aur-B stabilizes spindle microtubules in the egg extracts by inhibiting the catastrophe kinesin MCAK. Whether or not Aur-B plays a similar role in intact oocytes remains unknown. Here we have employed a dominant-negative Aur-B mutant (Aur-B122R, in which the ATP-binding lysine(122) is replaced with arginine) to investigate the function of Aur-B in spindle assembly in Xenopus oocytes undergoing meiosis. Overexpression of Aur-B122R results in short bipolar spindles or monopolar spindles, with higher concentrations of Aur-B122R producing mostly the latter. Simultaneous inhibition of MCAK translation in oocytes overexpressing Aur-B122R results in suppression of monopolar phenotype, suggesting that Aur-B regulates spindle bipolarity by inhibiting MCAK. Furthermore, recombinant MCAK-4A protein, which lacks all four Aur-B phosphoryaltion sites and is therefore insensitive to Aur-B inhibition but not wild-type MCAK, recapitulated the monopolar phenotype in the oocytes. These results suggest that in vertebrate oocytes that lack centrosomes, one major function of Aur-B is to stabilize chromosome-associated spindle microtubules to ensure spindle bipolarity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.