ArticleNature structural & molecular biology2012
Architecture of the RNA polymerase II preinitiation complex and mechanism of ATP-dependent promoter opening.
Article in Nature structural & molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed, 132 citations in OpenAlex.
- A transcription factor by any other name - function beyond DNA binding and transactivation.EMBO reports · 2026Review
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- The general transcription factors (GTFs) of RNA polymerase II and their roles in plant development and stress responses.Critical reviews in biochemistry and molecular biology · 2024Review
- DNA opening during transcription initiation by RNA polymerase II in atomic detail.Biophysical journal · 2022Article
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- Epigenetic memory contributing to the pathogenesis of AKI-to-CKD transition.Frontiers in molecular biosciences · 2022Review
- Nucleolar TFIIE plays a role in ribosomal biogenesis and performance.Nucleic acids research · 2021Article
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- Extracytoplasmic Function σ Factors as Tools for Coordinating Stress Responses.International journal of molecular sciences · 2021Review
- How to limit the speed of a motor: the intricate regulation of the XPB ATPase and translocase in TFIIH.Nucleic acids research · 2020Article
- Direct binding of TFEα opens DNA binding cleft of RNA polymerase.Nature communications · 2020Article
- Structural basis of the XPB helicase-Bax1 nuclease complex interacting with the repair bubble DNA.Nucleic acids research · 2020Article
- Article
- XPA: DNA Repair Protein of Significant Clinical Importance.International journal of molecular sciences · 2020Review
- High-resolution cryo-EM structures of TFIIH and their functional implications.Current opinion in structural biology · 2019Review
- Organization and regulation of gene transcription.Nature · 2019Review
- Transcription preinitiation complex structure and dynamics provide insight into genetic diseases.Nature structural & molecular biology · 2019Article
- TFIIE orchestrates the recruitment of the TFIIH kinase module at promoter before release during transcription.Nature communications · 2019Article
20 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Yeast RNA polymerase II (Pol II) general transcription factor TFIIE and the TFIIH subunit Ssl2 (yeast ortholog of mammalian XPB) function in the transition of the preinitiation complex (PIC) to the open complex. We show that the three TFIIE winged-helix (WH) domains form a heterodimer, with the Tfa1 (TFIIEα) WH binding the Pol II clamp and the Tfa2 (TFIIEβ) tandem WH domain encircling promoter DNA that becomes single-stranded in the open complex. Ssl2 lies adjacent to TFIIE, enclosing downstream promoter DNA. Unlike previous proposals, comparison of the PIC and open-complex models strongly suggests that Ssl2 promotes DNA opening by functioning as a double-stranded-DNA translocase, feeding 15 base pairs into the Pol II cleft. Right-handed threading of DNA through the Ssl2 binding groove, combined with the fixed position of upstream promoter DNA, leads to DNA unwinding and the open state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.