ArticleDevelopmental biology2012
cSrc is necessary for epididymal development and is incorporated into sperm during epididymal transit.
Article in Developmental biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 84 citations in OpenAlex.
- Immunoregulatory mechanisms between epithelial clear cells and mononuclear phagocytes in the epididymis.Andrology · 2024Pooled it
- Immunological dynamics of the epididymis: from experimental insights to clinical translation.Reproduction & fertility · 2026Review
- Engineered extracellular vesicles: a breakthrough approach to overcoming sperm cryopreservation challenges.Reproductive biology and endocrinology : RB&E · 2025Review
- Review
- The effect of epididymosomes on the development of frozen-thawed mouse spermatogonial stem cells after culture in a decellularized testicular scaffold and transplantation into azoospermic mice.Journal of assisted reproduction and genetics · 2024Article
- Exosomes: New regulators of reproductive development.Materials today. Bio · 2023Review
- Sperm acquire epididymis-derived proteins through epididymosomes.Human reproduction (Oxford, England) · 2022Article
- Soma-to-germline RNA communication.Nature reviews. Genetics · 2022Review
- The Mechanism and Experimental Validation of Forsythoside A in the Treatment of Male Infertility Were Analyzed Based on Network Pharmacology and Molecular Docking.Evidence-based complementary and alternative medicine : eCAM · 2022Article
- Maternal Protein Restriction Alters the Expression of Proteins Related to the Structure and Functioning of the Rat Offspring Epididymis in an Age-Dependent Manner.Frontiers in cell and developmental biology · 2022Article
- Sperm Heterogeneity Accounts for Sperm DNA Methylation Variations Observed in the Caput Epididymis, Independently From DNMT/TET Activities.Frontiers in cell and developmental biology · 2022Article
- An Update on Semen Physiology, Technologies, and Selection Techniques for the Advancement of In Vitro Equine Embryo Production: Section I.Animals : an open access journal from MDPI · 2021Review
- Caput Ligation Renders Immature Mouse Sperm Motile and Capable to Undergo cAMP-Dependent Phosphorylation.International journal of molecular sciences · 2021Article
- The transformative impact of extracellular vesicles on developing sperm.Reproduction & fertility · 2021Review
- Vacuolar H(+)-ATPase is not restricted to clear cells of the epididymal epithelium in cattle.Journal of animal science and technology · 2021Article
- Stability of the cytosine methylome during post-testicular sperm maturation in mouse.PLoS genetics · 2021Article
- Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis).Molecular reproduction and development · 2021Article
- The Role of Extracellular Vesicles in Sperm Function and Male Fertility.Sub-cellular biochemistry · 2021Review
- Article
- Novel role of proton-secreting epithelial cells in sperm maturation and mucosal immunity.Journal of cell science · 2019Article
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Authors and funding
10 authors at 5 institutions in 2 countries.
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Abstract
Changes that occur to mammalian sperm upon epididymal transit and maturation render these cells capable of moving progressively and capacitating. Signaling events leading to mammalian sperm capacitation depend on the modulation of proteins by phosphorylation and dephosphorylation cascades. Recent experiments have demonstrated that the Src family of kinases plays an important role in the regulation of these events. However, sperm from cSrc null mice display normal tyrosine phosphorylation associated with capacitation. We report here that, despite normal phosphorylation, sperm from cSrc null mice display a severe reduction in forward motility, and are unable to fertilize in vitro. Histological analysis of seminiferous tubules in the testes, caput and corpus epididymis do not reveal obvious defects. However, the cauda epididymis is significantly smaller, and expression of key transport proteins in the epithelial cells lining this region is reduced in cSrc null mice compared to wild type littermates. Although previously, we and others have shown the presence of cSrc in mature sperm from cauda epididymis, a closer evaluation indicates that this tyrosine kinase is not present in sperm from the caput epididymis, suggesting that this protein is acquired by sperm later during epididymal maturation. Consistent with this observation, cSrc is enriched in vesicles released by the epididymal epithelium known as epididymosomes. Altogether, these observations indicate that cSrc is essential for cauda epididymal development and suggest an essential role of this kinase in epididymal sperm maturation involving cSrc extracellular trafficking.
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