ReviewJournal of molecular biology2012
Mass spectrometry--from peripheral proteins to membrane motors.
Review in Journal of molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- The Adsorption of P2X2 Receptors Interacting with IgG Antibodies Revealed by Combined AFM Imaging and Mechanical Simulation.International journal of molecular sciences · 2023Article
- Electrospray ionization of native membrane proteins proceedsRSC advances · 2022Article
- Protein Lipidation Types: Current Strategies for Enrichment and Characterization.International journal of molecular sciences · 2022Review
- Charge Reduction of Membrane Proteins in Native Mass Spectrometry Using Alkali Metal Acetate Salts.Analytical chemistry · 2020Article
- Scratching the surface: native mass spectrometry of peripheral membrane protein complexes.Biochemical Society transactions · 2020Review
- LILBID and nESI: Different Native Mass Spectrometry Techniques as Tools in Structural Biology.Journal of the American Society for Mass Spectrometry · 2019Article
- Lipid binding attenuates channel closure of the outer membrane protein OmpF.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Unraveling self-assembly pathways of the 468-kDa proteolytic machine TET2.Science advances · 2017Article
- Combining Mass Spectrometry and X-Ray Crystallography for Analyzing Native-Like Membrane Protein Lipid Complexes.Frontiers in physiology · 2017Article
- Low Charge and Reduced Mobility of Membrane Protein Complexes Has Implications for Calibration of Collision Cross Section Measurements.Analytical chemistry · 2016Article
- Charge reduction stabilizes intact membrane protein complexes for mass spectrometry.Journal of the American Chemical Society · 2014Article
- Uncovering the stoichiometry of Pyrococcus furiosus RNase P, a multi-subunit catalytic ribonucleoprotein complex, by surface-induced dissociation and ion mobility mass spectrometry.Angewandte Chemie (International ed. in English) · 2014Article
- Defining the stoichiometry and cargo load of viral and bacterial nanoparticles by Orbitrap mass spectrometry.Journal of the American Chemical Society · 2014Article
- Characterization of unstable products of flavin- and pterin-dependent enzymes by continuous-flow mass spectrometry.Biochemistry · 2014Article
- Ion mobility-mass spectrometry of a rotary ATPase reveals ATP-induced reduction in conformational flexibility.Nature chemistry · 2014Article
- Hydrogen exchange mass spectrometry of functional membrane-bound chemotaxis receptor complexes.Biochemistry · 2013Article
- Native electrospray ionization and electron-capture dissociation for comparison of protein structure in solution and the gas phase.International journal of mass spectrometry · 2013Article
- Comparative cross-linking and mass spectrometry of an intact F-type ATPase suggest a role for phosphorylation.Nature communications · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
That membrane protein complexes could survive in the gas phase had always seemed impossible. The lack of chargeable residues, high hydrophobicity, and poor solubility and the vast excess of detergent contributed to the view that it would not be possible to obtain mass spectra of intact membrane complexes. With the recent success in recording mass spectra of these complexes, first from recombinant sources and later from the cellular environment, many surprising properties of these gas phase membrane complexes have been revealed. The first of these was that the interactions between membrane and soluble subunits could survive in vacuum, without detergent molecules adhering to the complex. The second unexpected feature was that their hydrophobicity and, consequently, lower charge state did not preclude ionization. The final surprising finding was that these gas phase membrane complexes carry with them lipids, bound specifically in subunit interfaces. This provides us with an opportunity to distinguish annular lipids that surround the membrane complexes, from structural lipids that have a role in maintaining structure and subunit interactions. In this perspective, we track these developments and suggest explanations for the various discoveries made during this research.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.