ArticleJournal of magnetic resonance (San Diego, Calif. : 1997)2012
Inter-spin distance determination using L-band (1-2 GHz) non-adiabatic rapid sweep electron paramagnetic resonance (NARS EPR).
Article in Journal of magnetic resonance (San Diego, Calif. : 1997), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Segmented-overlap Fourier filtering and averaging (SOFFA) approach to improve concentration sensitivity of magnetic resonance spectra.Magnetic resonance (Gottingen, Germany) · 2026Article
- A pressure-jump EPR system to monitor millisecond conformational exchange rates of spin-labeled proteins.Protein science : a publication of the Protein Society · 2024Article
- A pressure-jump EPR system to monitor millisecond conformational exchange rates of spin-labeled proteins.bioRxiv : the preprint server for biology · 2024Article
- Site-Directed Spin Labeling EPR for Studying Membrane Proteins.BioMed research international · 2018Review
- Autobiography of James S. Hyde.Applied magnetic resonance · 2017Article
- Rapid-scan EPR imaging.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2017Review
- A triarylmethyl spin label for long-range distance measurement at physiological temperatures using T1 relaxation enhancement.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2016Article
- Field-stepped direct detection electron paramagnetic resonance.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2015Article
- Spin-label CW microwave power saturation and rapid passage with triangular non-adiabatic rapid sweep (NARS) and adiabatic rapid passage (ARP) EPR spectroscopy.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2015Article
- A Straightforward Approach to the Analysis of Double Electron-Electron Resonance Data.Methods in enzymology · 2015Article
- EPR Methods for Biological Cu(II): L-Band CW and NARS.Methods in enzymology · 2015Article
- Long-range distance measurements in proteins at physiological temperatures using saturation recovery EPR spectroscopy.Journal of the American Chemical Society · 2014Article
- Moving difference (MDIFF) non-adiabatic rapid sweep (NARS) EPR of copper(II).Journal of magnetic resonance (San Diego, Calif. : 1997) · 2013Article
- Technological advances in site-directed spin labeling of proteins.Current opinion in structural biology · 2013Review
- Use of electron paramagnetic resonance to solve biochemical problems.Biochemistry · 2013Review
- Biophysical EPR Studies Applied to Membrane Proteins.Journal of physical chemistry & biophysicsArticle
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3 authors.
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Abstract
Site-directed spin-labeling electron paramagnetic resonance (SDSL EPR) provides insight into the local structure and motion of a spin probe strategically attached to a molecule. When a second spin is introduced to the system, macromolecular information can be obtained through measurement of inter-spin distances either by continuous wave (CW) or pulsed electron double resonance (ELDOR) techniques. If both methodologies are considered, inter-spin distances of 8-80 Å can be experimentally determined. However, there exists a region at the upper limit of the conventional X-band (9.5 GHz) CW technique and the lower limit of the four-pulse double electron-electron resonance (DEER) experiment where neither method is particularly reliable. The work presented here utilizes L-band (1.9 GHz) in combination with non-adiabatic rapid sweep (NARS) EPR to address this opportunity by increasing the upper limit of the CW technique. Because L-band linewidths are three to seven times narrower than those at X-band, dipolar broadenings that are small relative to the X-band inhomogeneous linewidth become observable, but the signal loss, due to the frequency dependence of the Boltzmann factor, has made L-band especially challenging. NARS has been shown to increase sensitivity by a factor of five, and overcomes much of this loss, making L-band distance determination more feasible. Two different systems are presented, and distances of 18-30 Å have been experimentally determined at physiologically relevant temperatures. Measurements are in excellent agreement with a helical model and values determined by DEER.
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