ArticleChemistry & biology2012
Visualization of lipid metabolism in the zebrafish intestine reveals a relationship between NPC1L1-mediated cholesterol uptake and dietary fatty acid.
Article in Chemistry & biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Genomic dissection of conserved transcriptional regulation in intestinal epithelial cells.PLoS biology · 2017Article
- The zebrafish goosepimples/myosin Vb mutant exhibits cellular attributes of human microvillus inclusion disease.Mechanisms of development · 2016Article
- Recent discoveries on absorption of dietary fat: Presence, synthesis, and metabolism of cytoplasmic lipid droplets within enterocytes.Biochimica et biophysica acta · 2016Review
- In Vivo Determination of Body Composition in Zebrafish (Danio rerio) by Quantitative Magnetic Resonance.Zebrafish · 2016Article
- Zebrafish: an important tool for liver disease research.Gastroenterology · 2015Review
- Arsenic-induced dyslipidemia in male albino rats: comparison between trivalent and pentavalent inorganic arsenic in drinking water.BMC pharmacology & toxicology · 2015Article
- Plexin D1 determines body fat distribution by regulating the type V collagen microenvironment in visceral adipose tissue.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Animal model of Sar1b deficiency presents lipid absorption deficits similar to Anderson disease.Journal of molecular medicine (Berlin, Germany) · 2015Article
- Whole-body imaging of a hypercholesterolemic female zebrafish by using synchrotron X-ray micro-CT.Zebrafish · 2015Article
- Studying lipoprotein trafficking in zebrafish, the case of chylomicron retention disease.Journal of molecular medicine (Berlin, Germany) · 2015Article
- High fat plus high cholesterol diet lead to hepatic steatosis in zebrafish larvae: a novel model for screening anti-hepatic steatosis drugs.Nutrition & metabolism · 2015Article
- Dietary cholesterol directly induces acute inflammasome-dependent intestinal inflammation.Nature communications · 2014Article
- Disruption of ldlr causes increased LDL-c and vascular lipid accumulation in a zebrafish model of hypercholesterolemia.Journal of lipid research · 2014Article
- Mitochondrial metabolism, reactive oxygen species, and macrophage function-fishing for insights.Journal of molecular medicine (Berlin, Germany) · 2014Review
- Synthesis of cholesterol analogues bearing BODIPY fluorophores by Suzuki or Liebeskind-Srogl cross-coupling and evaluation of their potential for visualization of cholesterol pools.Chembiochem : a European journal of chemical biology · 2014Article
- Could a swimming creature inform us on intestinal diseases? Lessons from zebrafish.Inflammatory bowel diseases · 2014Review
- Zebrafish models of dyslipidemia: relevance to atherosclerosis and angiogenesis.Translational research : the journal of laboratory and clinical medicine · 2014Review
- New insights into the molecular mechanism of intestinal fatty acid absorption.European journal of clinical investigation · 2013Review
- Microbiota regulate intestinal absorption and metabolism of fatty acids in the zebrafish.Cell host & microbe · 2012Article
Corrections and comments
- Correction · 2012-08-24Error in Text ·
- Retraction · 2015-09-17Error in Materials · Error in Methods · Error in Results and/or Conclusions ·
- Erratum issuedDosage error in article text
- Retracted
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The small intestine is the primary site of dietary lipid absorption in mammals. The balance of nutrients, microorganisms, bile, and mucus that determine intestinal luminal environment cannot be recapitulated ex vivo, thus complicating studies of lipid absorption. We show that fluorescently labeled lipids can be used to visualize and study lipid absorption in live zebrafish larvae. We demonstrate that the addition of a BODIPY-fatty acid to a diet high in atherogenic lipids enables imaging of enterocyte lipid droplet dynamics in real time. We find that a lipid-rich meal promotes BODIPY-cholesterol absorption into an endosomal compartment distinguishable from lipid droplets. We also show that dietary fatty acids promote intestinal cholesterol absorption by rapid re-localization of NPC1L1 to the intestinal brush border. These data illustrate the power of the zebrafish system to address longstanding questions in vertebrate digestive physiology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.