ArticleMicrobial cell factories2012
Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production.
Article in Microbial cell factories, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- Yarrowia lipolytica and Komagataella phaffii (Pichia pastoris) as Hosts for Heterologous Expression of Lipases.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Production system establishment and in vitro study of CD19/CD3ε bite antibody secreted from Pichia pastoris.Scientific reports · 2025Article
- Optimal fermentation conditions for growth and recombinant protein production inCurrent research in food science · 2024Article
- Artificial Diploid Escherichia coli by a CRISPR Chromosome-Doubling Technique.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- A New Expression System Based on PsychrotolerantInternational journal of molecular sciences · 2022Article
- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022Article
- What makes Komagataella phaffii non-conventional?FEMS yeast research · 2021Review
- Engineered Polyploid Yeast Strains Enable Efficient Xylose Utilization and Ethanol Production in Corn Hydrolysates.Frontiers in bioengineering and biotechnology · 2021Article
- Functional production of human antibody by the filamentous fungusFungal biology and biotechnology · 2020Article
- Review
- Genomic diversity and meiotic recombination among isolates of the biotech yeast Komagataella phaffii (Pichia pastoris).Microbial cell factories · 2019Article
- The Suitability of Orthogonal Hosts to Study Plant Cell Wall Biosynthesis.Plants (Basel, Switzerland) · 2019Review
- Creation of Stable Heterothallic Strains of Komagataella phaffii Enables Dissection of Mating Gene Regulation.Molecular and cellular biology · 2018Article
- Screening and identification of host proteins interacting with Toxoplasma gondii SAG2 by yeast two-hybrid assay.Parasites & vectors · 2017Article
- Molecular strategies to increase the levels of heterologous transcripts in Komagataella phaffii for protein production.Bioengineered · 2017Review
- Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.Nature communications · 2016Article
- Article
- Expression of recombinant antibodies.Frontiers in immunology · 2013Article
- A dual-mode surface display system for the maturation and production of monoclonal antibodies in glyco-engineered Pichia pastoris.PloS one · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundYeast mating provides an efficient means for strain and library construction. However, biotechnological applications of mating in the methylotrophic yeast Pichia pastoris have been hampered because of concerns about strain stability of P. pastoris diploids. The aim of the study reported here is to investigate heterologous protein expression in diploid P. pastoris strains and to evaluate diploid strain stability using high cell density fermentation processes.
resultsBy using a monoclonal antibody as a target protein, we demonstrate that recombinant protein production in both wild-type and glycoengineered P. pastoris diploids is stable and efficient during a nutrient rich shake flask cultivation. When diploid strains were cultivated under bioreactor conditions, sporulation was observed. Nevertheless, both wild-type and glycoengineered P. pastoris diploids showed robust productivity and secreted recombinant antibody of high quality. Specifically, the yeast culture maintained a diploid state for 240 h post-induction phase while protein titer and N-linked glycosylation profiles were comparable to that of a haploid strain expressing the same antibody. As an application of mating, we also constructed an antibody display library and used mating to generate novel full-length antibody sequences.
conclusionsTo the best of our knowledge, this study reports for the first time a comprehensive characterization of recombinant protein expression and fermentation using diploid P. pastoris strains. Data presented here support the use of mating for various applications including strain consolidation, variable-region glycosylation antibody display library, and process optimization.
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