ArticleTraffic (Copenhagen, Denmark)2012
Termination of isoform-selective Vps21/Rab5 signaling at endolysosomal organelles by Msb3/Gyp3.
Article in Traffic (Copenhagen, Denmark), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 60 citations in OpenAlex.
- Article
- Rethinking the Yeast Endomembrane System.Sub-cellular biochemistry · 2026Review
- A Saccharomyces cerevisiae knockout screen for genes critical for growth under sulfur- and nitrogen-limited conditions reveals intracellular sorting via vesicular transport systems.G3 (Bethesda, Md.) · 2025Article
- Role of Redox Homeostasis in the Communication Between Brain and Liver Through Extracellular Vesicles.Antioxidants (Basel, Switzerland) · 2024Review
- Studying the trafficking of labeled trodusquemine and its application as nerve marker for light-sheet and expansion microscopy.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- The entry of unclosed autophagosomes into vacuoles and its physiological relevance.PLoS genetics · 2022Article
- The MyLO CRISPR-Cas9 toolkit: a markerless yeast localization and overexpression CRISPR-Cas9 toolkit.G3 (Bethesda, Md.) · 2022Article
- Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase.G3 (Bethesda, Md.) · 2021Article
- Subunit exchange among endolysosomal tethering complexes is linked to contact site formation at the vacuole.Molecular biology of the cell · 2021Article
- Biogenesis of Extracellular Vesicles.Sub-cellular biochemistry · 2021Article
- High-throughput ultrastructure screening using electron microscopy and fluorescent barcoding.The Journal of cell biology · 2019Article
- Rab5 GTPases are required for optimal TORC2 function.The Journal of cell biology · 2019Article
- Rab5-mediated endosome formation is regulated at theCommunications biology · 2019Article
- Rab5-independent activation and function of yeast Rab7-like protein, Ypt7p, in the AP-3 pathway.PloS one · 2019Article
- Molecular control of Rab activity by GEFs, GAPs and GDI.Small GTPases · 2018Review
- Budding Yeast Has a Minimal Endomembrane System.Developmental cell · 2018Article
- Sec17 (α-SNAP) and an SM-tethering complex regulate the outcome of SNARE zippering in vitro and in vivo.eLife · 2017Article
- Regulation of Rab5 isoforms by transcriptional and post-transcriptional mechanisms in yeast.FEBS letters · 2017Article
- A Rab5 GTPase module is important for autophagosome closure.PLoS genetics · 2017Article
- Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast.Journal of visualized experiments : JoVE · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Traffic through endosomes and lysosomes is controlled by small G-proteins of the Rab5 and Rab7 families. Like humans, Saccharomyces cerevisiae has three Rab5s (Vps21, Ypt52 and Ypt53) and one Rab7 (Ypt7). Here, we elucidate the functional roles and regulation of the yeast Rab5s. Using GFP-tagged cargoes, a novel quantitative multivesicular body (MVB) sorting assay, and electron microscopy, we show that MVB biogenesis and thus MVB cargo sorting is severely impaired in vps21Δ ypt52Δ double mutants. Ypt53, the third Rab5 paralog, is hardly expressed during normal growth but its transcription is strongly induced by cellular stress through the calcineurin-Crz1 pathway. The requirement for Rab5 activity in stress tolerance facilitated identification of Msb3/Gyp3 as the principal Rab5 GAP (GTPase accelerating protein). In vitro GAP assays verified that Vps21 is a preferred Gyp3 target. Moreover, we demonstrate that Gyp3 spatially restricts active Vps21 to intermediate endosomal compartments by preventing Vps21 accumulation on lysosomal vacuoles. Gyp3, therefore, operates as a compartmental insulator that helps to define the spatial domain of Vps21 signaling in the endolysosomal pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.