ArticleLetters in applied microbiology2012
Development and evaluation of loop-mediated isothermal amplification assay for rapid and sensitive detection of canine parvovirus DNA directly in faecal specimens.
Article in Letters in applied microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
- Feline panleukopenia virus and canine parvovirus: development of two qPCR assays with high-resolution melting analysis and molecular epidemiology in dogs and cats from Northen Italy in 2017-2023.Frontiers in veterinary science · 2026Article
- Detection of canine parvovirus and feline panleukopenia virus in fecal samples by strand exchange amplification.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Article
- An Improved Polymerase Cross-Linking Spiral Reaction Assay for Rapid Diagnostic of Canine Parvovirus 2 Infection.Frontiers in veterinary science · 2020Article
- CRISPR-Cas13a mediated nanosystem for attomolar detection of canine parvovirus type 2.Chinese chemical letters = Zhongguo hua xue kuai bao · 2019Article
- A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China.Molecular and cellular probes · 2018Article
- Molecular characterisation of parvoviruses from domestic cats reveals emergence of newer variants in India.Journal of feline medicine and surgery · 2017Article
- A novel assay for detecting canine parvovirus using a quartz crystal microbalance biosensor.Journal of virological methods · 2015Article
- Visual detection of canine parvovirus based on loop-mediated isothermal amplification combined with enzyme-linked immunosorbent assay and with lateral flow dipstick.The Journal of veterinary medical science · 2014Article
- Phylogenetic analysis of canine parvovirus partial VP2 gene in India.Virus genes · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo develop a specific and highly sensitive loop-mediated isothermal amplification (LAMP) technique for the rapid detection of canine parvovirus (CPV) DNA directly in suspected faecal samples of dogs by employing a simple method of template preparation. METHODS AND
resultsLAMP reaction was developed by designing two sets of outer and inner primers, which target a total of six distinct regions on VP2 gene of CPV. The template DNA was prepared by a simple boiling and chilling method. Of the 140 faecal samples screened by the developed LAMP and the conventional PCR assays, 104 samples (74·28%) were found positive by LAMP, whereas 81 samples (57·85%) were found positive by PCR. The specificity of the LAMP assay was tested by cross-examination of common pathogens of dogs and further confirmed by sequencing. The detection limit of the LAMP was 0·0001 TCID₅₀ ml⁻¹, whereas the detection limit of the PCR was 1000 TCID₅₀ ml⁻¹.
conclusionsThe developed LAMP assay detects CPV DNA in faecal specimens directly within an hour by following a simple and rapid boiling and chilling method of template preparation. The result also shows that the developed LAMP assay is specific and highly sensitive in detecting CPV. SIGNIFICANCE AND IMPACT OF THE STUDY: The result indicates the potential usefulness of LAMP which is a simple, rapid, specific, highly sensitive and cost-effective field-based method for direct detection of CPV from the suspected faecal samples of dogs.
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