ArticleBMC genomics2012
Transcriptome characterization via 454 pyrosequencing of the annelid Pristina leidyi, an emerging model for studying the evolution of regeneration.
Article in BMC genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Spontaneous ectopic head formation enables reversal of the body axis polarity in microscopic flatworms.Proceedings. Biological sciences · 2025Article
- Annelid adult cell type diversity and their pluripotent cellular origins.Nature communications · 2024Article
- Cell Proliferation Indices in RegeneratingCells · 2023Article
- Distinct patterns of gene expression during regeneration and asexual reproduction in the annelid Pristina leidyi.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2022Article
- Morphological and molecular features of early regeneration in the marine annelid Ophryotrocha xiamen.Scientific reports · 2022Article
- Effects of GSK3β inhibition in the regeneration of Syllis malaquini (Syllidae, Annelida).Development genes and evolution · 2021Article
- Comparative Aspects of Annelid Regeneration: Towards Understanding the Mechanisms of Regeneration.Genes · 2021Review
- Injury-Induced Innate Immune Response During Segment Regeneration of the Earthworm,International journal of molecular sciences · 2021Article
- The transcriptome of anterior regeneration in earthworm Eudrilus eugeniae.Molecular biology reports · 2021Article
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- Regeneration mechanisms in Syllidae (Annelida).Regeneration (Oxford, England) · 2018Review
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- A transcriptional blueprint for a spiral-cleaving embryo.BMC genomics · 2016Article
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- Characterization of Liaoning cashmere goat transcriptome: sequencing, de novo assembly, functional annotation and comparative analysis.PloS one · 2013Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe naid annelids contain a number of species that vary in their ability to regenerate lost body parts, making them excellent candidates for evolution of regeneration studies. However, scant sequence data exists to facilitate such studies. We constructed a cDNA library from the naid Pristina leidyi, a species that is highly regenerative and also reproduces asexually by fission, using material from a range of regeneration and fission stages for our library. We then sequenced the transcriptome of P. leidyi using 454 technology.
results454 sequencing produced 1,550,174 reads with an average read length of 376 nucleotides. Assembly of 454 sequence reads resulted in 64,522 isogroups and 46,679 singletons for a total of 111,201 unigenes in this transcriptome. We estimate that over 95% of the transcripts in our library are present in our transcriptome. 17.7% of isogroups had significant BLAST hits to the UniProt database and these include putative homologs of a number of genes relevant to regeneration research. Although many sequences are incomplete, the mean sequence length of transcripts (isotigs) is 707 nucleotides. Thus, many sequences are large enough to be immediately useful for downstream applications such as gene expression analyses. Using in situ hybridization, we show that two Wnt/β-catenin pathway genes (homologs of frizzled and β-catenin) present in our transcriptome are expressed in the regeneration blastema of P. leidyi, demonstrating the usefulness of this resource for regeneration research.
conclusions454 sequencing is a rapid and efficient approach for identifying large numbers of genes in an organism that lacks a sequenced genome. This transcriptome dataset will be a valuable resource for molecular analyses of regeneration in P. leidyi and will serve as a starting point for comparisons to non-regenerating naids. It also contributes significantly to the still limited genomic resources available for annelids and lophotrochozoans more generally.
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