ArticleBMC biotechnology2012
pMAA-Red: a new pPZP-derived vector for fast visual screening of transgenic Arabidopsis plants at the seed stage.
Article in BMC biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 26 citations in OpenAlex.
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- Functional intron-derived miRNAs and host-gene expression in plants.Plant methods · 2018Article
- Transgenic Strategies for Enhancement of Nematode Resistance in Plants.Frontiers in plant science · 2017Review
- Evolutionary transgenomics: prospects and challenges.Frontiers in plant science · 2015Article
- Self-processing of a barley subtilase expressed in E. coli.Protein expression and purification · 2014Article
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- pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants.PloS one · 2014Article
- Overexpression of the transcription factor RAP2.6 leads to enhanced callose deposition in syncytia and enhanced resistance against the beet cyst nematode Heterodera schachtii in Arabidopsis roots.BMC plant biology · 2013Article
- Comparison of Expression Vectors for Transient Expression of Recombinant Proteins in Plants.Plant molecular biology reporter · 2013Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe production of transgenic plants, either for the overproduction of the protein of interest, for promoter: reporter lines, or for the downregulation of genes is an important prerequisite in modern plant research but is also very time-consuming.
resultsWe have produced additions to the pPZP family of vectors. Vector pPZP500 (derived from pPZP200) is devoid of NotI sites and vector pPZP600 (derived from pPZP500) contains a bacterial kanamycin resistance gene. Vector pMAA-Red contains a Pdf2.1: DsRed marker and a CaMV:: GUS cassette within the T-DNA and is useful for the production of promoter: GUS lines and overexpression lines. The Pdf2.1 promoter is expressed in seeds and syncytia induced by the beet cyst nematode Heterodera schachti in Arabidopsis roots. Transgenic seeds show red fluorescence which can be used for selection and the fluorescence level is indicative of the expression level of the transgene. The advantage is that plants can be grown on soil and that expression of the marker can be directly screened at the seed stage which saves time and resources. Due to the expression of the Pdf2.1: DsRed marker in syncytia, the vector is especially useful for the expression of a gene of interest in syncytia.
conclusionsThe vector pMAA-Red allows for fast and easy production of transgenic Arabidopsis plants with a strong expression level of the gene of interest.
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