ArticlePloS one2012
A novel porcine in vitro model of the blood-cerebrospinal fluid barrier with strong barrier function.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Primary cell and tissue cultures of human choroid plexus epithelial cells.Frontiers in cellular neuroscience · 2026Article
- Microphysiological modeling of the Blood-CSF barrier for biopharmaceutical transport and sequestration: evaluation of PEG-induced accumulation and vacuolation in the choroid plexus epithelium.Frontiers in pharmacology · 2026Article
- In vitro models of the choroid plexus and the blood-cerebrospinal fluid barrier: advances, applications, and perspectives.Human cell · 2024Review
- Characterization of TRPV4-mediated signaling pathways in an optimized human choroid plexus epithelial cell line.American journal of physiology. Cell physiology · 2022Article
- Following Ussing's legacy: from amphibian models to mammalian kidney and brain.American journal of physiology. Cell physiology · 2022Review
- In Vitro Models of the Blood-Cerebrospinal Fluid Barrier and Their Applications in the Development and Research of (Neuro)Pharmaceuticals.Pharmaceutics · 2022Review
- Porcine choroid plexus-Riems cell line demonstrates altered polarization of transport proteins compared with the native epithelium.American journal of physiology. Cell physiology · 2022Article
- Experimental approaches for manipulating choroid plexus epithelial cells.Fluids and barriers of the CNS · 2022Review
- Selenium at theFrontiers in neuroscience · 2021Review
- Polar Infection of Echovirus-30 Causes Differential Barrier Affection and Gene Regulation at the Blood-Cerebrospinal Fluid Barrier.International journal of molecular sciences · 2020Article
- Localization of ZIP14 and ZIP8 in HIBCPP Cells.Brain sciences · 2020Article
- Role of Bacterial and Host DNases on Host-Pathogen Interaction duringInternational journal of molecular sciences · 2020Article
- Cytokine and inflammatory mediator effects on TRPV4 function in choroid plexus epithelial cells.American journal of physiology. Cell physiology · 2019Article
- Optimized cultivation of porcine choroid plexus epithelial cells, a blood-cerebrospinal fluid barrier model, for studying granulocyte transmigration.Laboratory investigation; a journal of technical methods and pathology · 2019Article
- Activation of TRPV4 stimulates transepithelial ion flux in a porcine choroid plexus cell line.American journal of physiology. Cell physiology · 2018Article
- Experimental PorcineMediators of inflammation · 2017Article
- Neuroinvasion and Inflammation in Viral Central Nervous System Infections.Mediators of inflammation · 2016Review
- Characterization of efflux transport proteins of the human choroid plexus papilloma cell line HIBCPP, a functional in vitro model of the blood-cerebrospinal fluid barrier.Pharmaceutical research · 2015Article
- Brain barriers: Crosstalk between complex tight junctions and adherens junctions.The Journal of cell biology · 2015Review
- The choroid plexus-a multi-role player during infectious diseases of the CNS.Frontiers in cellular neuroscience · 2015Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epithelial cells of the plexus choroideus form the structural basis of the blood-cerebrospinal fluid barrier (BCSFB). In vitro models of the BCSFB presenting characteristics of a functional barrier are of significant scientific interest as tools for examination of BCSFB function. Due to a lack of suitable cell lines as in vitro models, primary porcine plexus epithelial cells were subjected to a series of selective cultivation steps until a stable continuous subcultivatable epithelial cell line (PCP-R) was established. PCP-R cells grow in a regular polygonal pattern with a doubling time of 28-36 h. At a cell number of 1.5×10(5) in a 24-well plate confluence is reached in 56-72 h. Cells are cytokeratin positive and chromosomal analysis revealed 56 chromosomes at peak (84th subculture). Employing reverse transcription PCR mRNA expression of several transporters and components of cell junctions could be detected. The latter includes tight junction components like Claudin-1 and -3, ZO-1, and Occludin, and the adherens junction protein E-cadherin. Cellular localization studies of ZO-1, Occludin and Claudin-1 by immunofluorescence and morphological analysis by electron microscopy demonstrated formation of a dense tight junction structure. Importantly, when grown on cell culture inserts PCP-R developed typical characteristics of a functional BCSFB including high transepithelial electrical resistance above 600 Ω×cm(2) as well as low permeability for macromolecules. In summary, our data suggest the PCP-R cell line as a suitable in vitro model of the porcine BCSFB.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.