ArticlePloS one2012
Fluorescence-based phenotypic selection allows forward genetic screens in haploid human cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Genome-wide forward genetic screening to identify receptors and proteins mediating nanoparticle uptake and intracellular processing.Nature nanotechnology · 2024Article
- Proteomic discovery of chemical probes that perturb protein complexes in human cells.Molecular cell · 2023Article
- Article
- Contribution of the clathrin adaptor AP-1 subunit µ1 to acidic cluster protein sorting.The Journal of cell biology · 2017Article
- Article
- A Genetic Screen Identifies a Critical Role for the WDR81-WDR91 Complex in the Trafficking and Degradation of Tetherin.Traffic (Copenhagen, Denmark) · 2016Article
- Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens.Nature communications · 2016Article
- Identification of Genes That Modulate Susceptibility to Formaldehyde and Imatinib by Functional Genomic Screening in Human Haploid KBM7 Cells.Toxicological sciences : an official journal of the Society of Toxicology · 2016Article
- Large-Scale Single Guide RNA Library Construction and Use for CRISPR-Cas9-Based Genetic Screens.Cold Spring Harbor protocols · 2016Article
- Hunting Viral Receptors Using Haploid Cells.Annual review of virology · 2015Review
- GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.Science (New York, N.Y.) · 2015Article
- The binding of TAPBPR and Tapasin to MHC class I is mutually exclusive.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Tapasin-related protein TAPBPR is an additional component of the MHC class I presentation pathway.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Haploid genetic screens identify an essential role for PLP2 in the downregulation of novel plasma membrane targets by viral E3 ubiquitin ligases.PLoS pathogens · 2013Article
- A reporter screen in a human haploid cell line identifies CYLD as a constitutive inhibitor of NF-κB.PloS one · 2013Article
- Functional genomic screening approaches in mechanistic toxicology and potential future applications of CRISPR-Cas9.Mutation research. Reviews in mutation researchReview
- Dissecting mammalian immunity through mutation.Immunology and cell biologyReview
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The isolation of haploid cell lines has recently allowed the power of forward genetic screens to be applied to mammalian cells. The interest in applying this powerful genetic approach to a mammalian system is only tempered by the limited utility of these screens, if confined to lethal phenotypes. Here we expand the scope of these approaches beyond live/dead screens and show that selection for a cell surface phenotype via fluorescence-activated cell sorting can identify the key molecules in an intracellular pathway, in this case MHC class I antigen presentation. Non-lethal haploid genetic screens are widely applicable to identify genes involved in essentially any cellular pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.