ArticlePloS one2012
Temporal stability of epigenetic markers: sequence characteristics and predictors of short-term DNA methylation variations.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Epigenetics and human obesity.International journal of obesity (2005) · 2015Pooled it
- Short-term diesel exhaust inhalation in a controlled human crossover study is associated with changes in DNA methylation of circulating mononuclear cells in asthmatics.Particle and fibre toxicology · 2014Trial
- DNA methylation-based surrogate markers of C-reactive protein and their associations with health-related traits.GeroScience · 2026Article
- The Evolving Landscape of Clinical Aging Clocks: From Epigenetic to Multi-Omics Integration.Aging cell · 2026Review
- Estimating population structure using epigenome-wide methylation data.Briefings in bioinformatics · 2026Article
- Estimating population structure using epigenome-wide methylation data.medRxiv : the preprint server for health sciences · 2025Article
- Methylation risk score of C-reactive protein associates sleep health with related health outcomes.Communications biology · 2025Article
- Epigenetic biomarkers of socioeconomic status are associated with age-related chronic diseases and mortality in older adults.PNAS nexus · 2025Article
- Identification of Key Genes Correlated with Economic Trait Superiorities and Their SNP Screening Through Transcriptome Comparisons, WGCNA and Pearson Correlation Coefficient in the Sea Cucumber Apostichopus Japonicus.Marine biotechnology (New York, N.Y.) · 2024Article
- Stability of Blood DNA Methylation Across Two Timepoints in Three Cohorts.Biomedicines · 2024Article
- DNA Methylation at C-Reactive Protein-Associated CpG Sites May Mediate the Pathway Between Educational Attainment and Cognition.The journals of gerontology. Series A, Biological sciences and medical sciences · 2024Article
- Particulate matter-induced hypomethylation of Alu and LINE1 in normal human bronchial epithelial cells and epidermal keratinocytes.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2022Article
- Long-term effects of wildfire smoke exposure during early life on the nasal epigenome in rhesus macaques.Environment international · 2022Article
- Longitudinal DNA methylation dynamics as a practical indicator in clinical epigenetics.Clinical epigenetics · 2021Article
- DNA Methylation and Protein Markers of Chronic Inflammation and Their Associations With Brain and Cognitive Aging.Neurology · 2021Article
- Article
- Ten Years of EWAS.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Longitudinal analysis of individual cfDNA methylome patterns in metastatic prostate cancer.Clinical epigenetics · 2021Article
- Structural brain correlates of serum and epigenetic markers of inflammation in major depressive disorder.Brain, behavior, and immunity · 2021Article
- Characterisation of an inflammation-related epigenetic score and its association with cognitive ability.Clinical epigenetics · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
backgroundDNA methylation is an epigenetic mechanism that has been increasingly investigated in observational human studies, particularly on blood leukocyte DNA. Characterizing the degree and determinants of DNA methylation stability can provide critical information for the design and conduction of human epigenetic studies.
methodsWe measured DNA methylation in 12 gene-promoter regions (APC, p16, p53, RASSF1A, CDH13, eNOS, ET-1, IFNγ, IL-6, TNFα, iNOS, and hTERT) and 2 of non-long terminal repeat elements, i.e., L1 and Alu in blood samples obtained from 63 healthy individuals at baseline (Day 1) and after three days (Day 4). DNA methylation was measured by bisulfite-PCR-Pyrosequencing. We calculated intraclass correlation coefficients (ICCs) to measure the within-individual stability of DNA methylation between Day 1 and 4, subtracted of pyrosequencing error and adjusted for multiple covariates.
resultsMethylation markers showed different temporal behaviors ranging from high (IL-6, ICC = 0.89) to low stability (APC, ICC = 0.08) between Day 1 and 4. Multiple sequence and marker characteristics were associated with the degree of variation. Density of CpG dinucleotides nearby the sequence analyzed (measured as CpG(o/e) or G+C content within ±200 bp) was positively associated with DNA methylation stability. The 3' proximity to repeat elements and range of DNA methylation on Day 1 were also positively associated with methylation stability. An inverted U-shaped correlation was observed between mean DNA methylation on Day 1 and stability.
conclusionsThe degree of short-term DNA methylation stability is marker-dependent and associated with sequence characteristics and methylation levels.
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