ArticlePlant physiology2012
Ectopic expression of Rubisco subunits in maize mesophyll cells does not overcome barriers to cell type-specific accumulation.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Laboratory evolution of Rubisco solubility and catalytic switches to enhance plant productivity.Nature plants · 2025Article
- Compartmentation of photosynthesis gene expression in C4 maize depends on time of day.Plant physiology · 2023Article
- The small subunit of Rubisco and its potential as an engineering target.Journal of experimental botany · 2023Review
- Enhancing crop yield by using Rubisco activase to improve photosynthesis under elevated temperatures.Stress biology · 2021Review
- The state of oligomerization of Rubisco controls the rate of synthesis of the Rubisco large subunit in Chlamydomonas reinhardtii.The Plant cell · 2021Article
- Rubisco feedback loop: control by epistasy of synthesis governs large subunit biosynthesis.The Plant cell · 2021Article
- Genome-Wide Characterization and Expression Analysis Provide Basis to the Biological Function of Cotton FBA Genes.Frontiers in plant science · 2021Article
- Modifying Plant Photosynthesis and Growth via Simultaneous Chloroplast Transformation of Rubisco Large and Small Subunits.The Plant cell · 2020Article
- Increased Rubisco content in maize mitigates chilling stress and speeds recovery.Plant biotechnology journal · 2020Article
- Cytological evidence of BSD2 functioning in both chloroplast division and dimorphic chloroplast formation in maize leaves.BMC plant biology · 2020Article
- Genetic determinants controlling maize rubisco activase gene expression and a comparison with rice counterparts.BMC plant biology · 2019Article
- Role of ClpP in the Biogenesis and Degradation of RuBisCO and ATP Synthase inPlants (Basel, Switzerland) · 2019Article
- The Rubisco Chaperone BSD2 May Regulate Chloroplast Coverage in Maize Bundle Sheath Cells.Plant physiology · 2017Article
- Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis.The New phytologist · 2017Article
- Post-transcriptional regulation of photosynthetic genes is a key driver of C4 leaf ontogeny.Journal of experimental botany · 2017Article
- Phaseolin expression in tobacco chloroplast reveals an autoregulatory mechanism in heterologous protein translation.Plant biotechnology journal · 2016Article
- Cosuppression of RBCS3B in Arabidopsis leads to severe photoinhibition caused by ROS accumulation.Plant cell reports · 2014Article
- Exploring mechanisms linked to differentiation and function of dimorphic chloroplasts in the single cell C4 species Bienertia sinuspersici.BMC plant biology · 2014Article
- A novel RNA binding protein affects rbcL gene expression and is specific to bundle sheath chloroplasts in C4 plants.BMC plant biology · 2013Article
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In maize (Zea mays), Rubisco accumulates in bundle sheath but not mesophyll chloroplasts, but the mechanisms that underlie cell type-specific expression are poorly understood. To explore the coordinated expression of the chloroplast rbcL gene, which encodes the Rubisco large subunit (LS), and the two nuclear RBCS genes, which encode the small subunit (SS), RNA interference was used to reduce RBCS expression. This resulted in Rubisco deficiency and was correlated with translational repression of rbcL. Thus, as in C3 plants, LS synthesis depends on the presence of its assembly partner SS. To test the hypothesis that the previously documented transcriptional repression of RBCS in mesophyll cells is responsible for repressing LS synthesis in mesophyll chloroplasts, a ubiquitin promoter-driven RBCS gene was expressed in both bundle sheath and mesophyll cells. This did not lead to Rubisco accumulation in the mesophyll, suggesting that LS synthesis is impeded even in the presence of ectopic SS expression. To attempt to bypass this putative mechanism, a ubiquitin promoter-driven nuclear version of the rbcL gene was created, encoding an epitope-tagged LS that was expressed in the presence or absence of the Ubi-RBCS construct. Both transgenes were robustly expressed, and the tagged LS was readily incorporated into Rubisco complexes. However, neither immunolocalization nor biochemical approaches revealed significant accumulation of Rubisco in mesophyll cells, suggesting a continuing cell type-specific impairment of its assembly or stability. We conclude that additional cell type-specific factors limit Rubisco expression to bundle sheath chloroplasts.
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