ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2012
Epithelial-mesenchymal transition and fibrosis are mutually exclusive reponses in shear-activated proximal tubular epithelial cells.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Emetine dihydrochloride alleviated radiation-induced lung injury through inhibiting EMT.Journal of cellular and molecular medicine · 2023Article
- Three dimensional modeling of biologically relevant fluid shear stress in human renal tubule cells mimics in vivo transcriptional profiles.Scientific reports · 2021Article
- The protective effects of granulocyte-macrophage colony-stimulating factor against radiation-induced lung injury.Translational lung cancer research · 2020Article
- A human proximal tubule-on-a-chip to study renal disease and toxicity.Biomicrofluidics · 2019Article
- Comprehensive transcriptome analysis of fluid shear stress altered gene expression in renal epithelial cells.Journal of cellular physiology · 2018Article
- Fluid Shear Stress Promotes Autophagy in Hepatocellular Carcinoma Cells.International journal of biological sciences · 2018Article
- Polydatin alleviated radiation-induced lung injury through activation of Sirt3 and inhibition of epithelial-mesenchymal transition.Journal of cellular and molecular medicine · 2017Article
- Fluid shear stress-induced TGF-β/ALK5 signaling in renal epithelial cells is modulated by MEK1/2.Cellular and molecular life sciences : CMLS · 2017Article
- Quantitative liver proteomics identifies FGF19 targets that couple metabolism and proliferation.PloS one · 2017Article
- Bioprinting of 3D Convoluted Renal Proximal Tubules on Perfusable Chips.Scientific reports · 2016Article
- Article
- Sesamin Ameliorates High-Fat Diet-Induced Dyslipidemia and Kidney Injury by Reducing Oxidative Stress.Nutrients · 2016Article
- The network of epithelial-mesenchymal transition: potential new targets for tumor resistance.Journal of cancer research and clinical oncology · 2015Review
- Shear Stress-Induced Alteration of Epithelial Organization in Human Renal Tubular Cells.PloS one · 2015Article
- Article
- Effects of Chinese Medicine Tong xinluo on Diabetic Nephropathy via Inhibiting TGF- β 1-Induced Epithelial-to-Mesenchymal Transition.Evidence-based complementary and alternative medicine : eCAM · 2014Article
- Notch4-dependent antagonism of canonical TGF-β1 signaling defines unique temporal fluctuations of SMAD3 activity in sheared proximal tubular epithelial cells.American journal of physiology. Renal physiology · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Renal fibrosis (RF) is thought to be a direct consequence of dedifferentiation of resident epithelial cells via an epithelial-mesenchymal transition (EMT). Increased glomerular flow is a critical initiator of fibrogenesis. Yet, the responses of proximal tubular epithelial cells (PTECs) to fluid flow remain uncharacterized. Here, we investigate the effects of pathological shear stresses on the development of fibrosis in PTECs. Our data reveal that type I collagen accumulation in shear-activated PTECs is accompanied by a ∼40-60% decrease in cell motility, thus excluding EMT as a relevant pathological process. In contrast, static incubation of PTECs with TGFβ1 increases cell motility by ∼50%, and induces stable expression of key mesenchymal markers, including Snail1, N-cadherin, and vimentin. Ectopic expression of TGFβ1 in shear-activated PTECs fails to induce EMT-associated changes but abrogates collagen accumulation via SMAD2-dependent mechanisms. Shear-mediated inhibition of EMT occurs via cyclic oscillations in both ERK2 activity and downstream expression of EMT genes. A constitutive ERK2 mutant induces stable expression of Snail1, N-cadherin, and vimentin, and increases cell motility in shear-activated PTECs by 250% without concomitant collagen deposition. Collectively, our data reveal that RF not only occurs without EMT but also that these two responses represent mutually exclusive cell fates.
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