ArticleAmerican journal of physiology. Cell physiology2012
Mechanisms and roles of muscarinic activation in guinea-pig adrenal medullary cells.
Article in American journal of physiology. Cell physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 24 citations in OpenAlex.
- Phospholipase C-ε defines a PACAP-stimulated pathway for secretion in the chromaffin cell.Journal of neuroendocrinology · 2023Article
- Expression of p11 and Heteromeric TASK Channels in Rat Carotid Body Glomus Cells and Nerve Growth Factor-differentiated PC12 Cells.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Article
- TASK channels: channelopathies, trafficking, and receptor-mediated inhibition.Pflugers Archiv : European journal of physiology · 2020Review
- Muscarinic receptors in adrenal chromaffin cells: physiological role and regulation of ion channels.Pflugers Archiv : European journal of physiology · 2018Review
- Molecular mechanism for muscarinic MThe Journal of physiology · 2017Article
- Two-pore domain potassium channels in the adrenal cortex.Pflugers Archiv : European journal of physiology · 2015Review
- Identification of muscarinic receptor subtypes involved in catecholamine secretion in adrenal medullary chromaffin cells by genetic deletion.British journal of pharmacology · 2015Article
- Nerve growth factor-induced endocytosis of TWIK-related acid-sensitive K⁺ 1 channels in adrenal medullary cells and PC12 cells.Pflugers Archiv : European journal of physiology · 2013Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Muscarinic receptors are expressed in the adrenal medullary (AM) cells of various mammals, but their physiological roles are controversial. Therefore, the ionic mechanism for muscarinic receptor-mediated depolarization and the role of muscarinic receptors in neuronal transmission were investigated in dissociated guinea-pig AM cells and in the perfused guinea-pig adrenal gland. Bath application of muscarine induced an inward current at -60 mV. This inward current was partially suppressed by quinine with an IC(50) of 6.1 μM. The quinine-insensitive component of muscarine-induced currents changed the polarity at -78 mV and was inhibited by bupivacaine, a TWIK-related acid-sensitive K(+) (TASK) channel inhibitor. Conversely, the current-voltage relationship for the bupivacaine-insensitive component of muscarine currents showed a reversal potential of -5 mV and a negative slope below -40 mV. External application of La(3+) had a double action on muscarine currents of both enhancement and suppression. Immunoblotting and immunocytochemistry revealed expression of TASK1 channels and cononical transient receptor potential channels 1, 4, 5, and 7 in guinea-pig AM cells. Retrograde application of atropine reversibly suppressed transsynaptically evoked catecholamine secretion from the adrenal gland. The results indicate that muscarinic receptor stimulation in guinea-pig AM cells induces depolarization through inhibition of TASK channels and activation of nonselective cation channels and that muscarinic receptors are involved in neuronal transmission from the splanchnic nerve.
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