ArticleJournal of virological methods2012
A plate-based high-throughput assay for virus stability and vaccine formulation.
Article in Journal of virological methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
65 citing papers in PubMed.
- Enhancing foot-and-mouth disease virus thermostability with a VP1 substitution selected by host HSP60 inhibition.Applied microbiology and biotechnology · 2026Article
- Human alpha-defensin 5 stabilizes the enterovirus A71 capsid and blocks infection.bioRxiv : the preprint server for biology · 2026Article
- Structural and functional mapping of protective human monoclonal antibodies against enterovirus A71.Science advances · 2026Article
- Article
- New rhinovirus uncoating intermediate reveals how sodium versus potassium ions influence RNA release.Scientific reports · 2025Article
- Article
- Enterovirus-like particles encapsidate RNA and exhibit decreased stability due to lack of maturation.PLoS pathogens · 2025Article
- Plate-Based High-Throughput Fluorescence Assay for Assessing Enveloped Virus Integrity.Biomacromolecules · 2024Article
- Article
- Highly stableFrontiers in bioengineering and biotechnology · 2024Article
- A tradeoff between enterovirus A71 particle stability and cell entry.Nature communications · 2023Article
- Substitution of Coxsackievirus A16 VP1 BC and EF Loop Altered the Protective Immune Responses in Chimera Enterovirus A71.Vaccines · 2023Article
- Stabilization of the Quadruplex-Forming G-Rich Sequences in the Rhinovirus Genome Inhibits Uncoating-Role of NaViruses · 2023Article
- Thermostable negative-marker foot-and-mouth disease virus serotype O induces protective immunity in guinea pigs.Applied microbiology and biotechnology · 2023Article
- Crosstalk between imbalanced gut microbiota caused by antibiotic exposure and rotavirus replication in the intestine.Heliyon · 2023Article
- Discovery and Optimization of Quinoline Analogues as Novel Potent Antivirals against Enterovirus D68.Journal of medicinal chemistry · 2022Article
- Article
- Enhancing the Stability of Bacteriophages Using Physical, Chemical, and Nano-Based Approaches: A Review.Pharmaceutics · 2022Review
- Membrane Interactions and Uncoating of Aichi Virus, a Picornavirus That Lacks a VP4.Journal of virology · 2022Article
- Exploring divalent conjugates of 5-RSC advances · 2022Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Standard methods for assessing the thermal stability of viruses can be time consuming and rather qualitative yet such data is a necessary requisite for vaccine formulation. In this study a novel plate-based thermal scanning assay for virus particle stability has been developed (PaSTRy: Particle Stability Thermal Release Assay). Two environment-sensitive fluorescent dyes, with non-overlapping emission spectra and different affinities, are used to accrue simultaneously independent data for the overall stability of the virus capsid, as judged by the exposure of the genome, and for capsid protein stability according to the exposure of hydrophobic side chains which are normally buried. This offers a fast and efficient high-throughput method to optimise vaccine formulation and to investigate the processes of virus uncoating.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.